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土耳其凡省流浪狗感染情况的患病率及基因分型初步研究。

Preliminary Studies on the Prevalence and Genotyping of Infection in Stray Dogs in Van Province, Turkey.

作者信息

Oguz Bekir, Ozdal Nalan, Kilinc Ozlem Orunc, Serdar Deger M

机构信息

Department of Parasitology, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, 65040, Turkey.

Ozalp Vocational High School, Van Yuzuncu Yil University, Van, 65800, Turkey.

出版信息

J Vet Res. 2018 Dec 31;62(4):497-502. doi: 10.2478/jvetres-2018-0061. eCollection 2018 Dec.

Abstract

INTRODUCTION

is a zoonotic helminth of the family living in the small intestines of dogs. The hydatid cyst, which is the larval form of this parasite, is observed in sheep, goat, cattle, and many other organisms including humans. It causes a disease called cystic echinococcosis. Identification of strains of in dogs is critical in parasite control and eradication where possible. This study aims to determine the genotype of eggs and prevalence of this parasite in the faeces of dogs in the Van Province using the copro-PCR method.

MATERIAL AND METHODS

This study was conducted between 2015 and 2016 on the faeces obtained from 100 stray dogs from different parts of the Van Province. The coprological examination was conducted using the formalin-ether concentration method.

RESULTS

eggs were found in 10 (10%) out of 100 faecal samples. was detected in 4 out of 10 of these (40%) infected samples. Sequence analysis of positive amplicons obtained from PCR showed that there were sheep strains (G1).

CONCLUSION

Dogs in Van area are primarily infected with the livestock genotype of , which is thought to be a potential zoonotic threat to humans.

摘要

引言

是一种生活在狗小肠内的 科人畜共患蠕虫。这种寄生虫的幼虫形式即包虫囊肿,在绵羊、山羊、牛以及包括人类在内的许多其他生物体内都有发现。它会引发一种名为囊性棘球蚴病的疾病。在可能的情况下,识别狗体内的 菌株对于寄生虫的控制和根除至关重要。本研究旨在使用粪便聚合酶链反应(copro-PCR)方法确定凡省狗粪便中 虫卵的基因型以及这种寄生虫的流行情况。

材料与方法

本研究于2015年至2016年期间,对从凡省不同地区采集的100只流浪狗的粪便进行了研究。粪便学检查采用福尔马林 - 乙醚浓缩法。

结果

在100份粪便样本中,有10份(10%)检测到 虫卵。在这10份受感染样本中的4份(40%)检测到了 。对PCR获得的阳性扩增子进行序列分析表明,存在绵羊菌株(G1)。

结论

凡省的狗主要感染的是 的家畜基因型,这被认为是对人类潜在的人畜共患威胁。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9fb/6364166/7537bb2f95f5/jvetres-62-497-g001.jpg

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