伊朗草原蝰蛇毒对人脐静脉内皮细胞的细胞毒性作用
Cytotoxic Effect of Iranian Vipera lebetina Snake Venom on HUVEC Cells.
作者信息
Kakanj Maryam, Ghazi-Khansari Mahmoud, Zare Mirakabadi Abbas, Daraei Bahram, Vatanpour Hossein
机构信息
Toxicology and Pharmacology Department, School of Pharmacy, Medical Science University of Shahid Beheshti, Tehran, Iran.
Pharmacology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Iran J Pharm Res. 2015 Winter;14(Suppl):109-14.
OBJECTIVE
Envenomation by heamotoxic snakes constituted a critical health occurrence in the world. Bleeding is the most sever consequence following snake bite with viperid and crothalid snakes. It is believed that the degradation of vascular membrane caused hemorrhage; in contrast, some suggested that direct cytotoxicity has role in endothelial cell disturbances. This study was carried out to evaluate the direct toxicity effect of V. lebetina crude venom on Human Umbilical Vein Endothelial Cells (HUVECs).
METHODS
The effect of V. lebetina snake venom on HUVECs growth inhibition was determined by MTT assay and neutral red uptake assay. The integrity of cell membrane through LDH release was measured with the Cytotoxicity Detection Kit. Morphological changes of endothelial cells were also evaluated using a phase contrast microscope.
RESULT
In MTT assay, crude venom showed a cytotoxic effect on endothelial cells which was confirmed by the effect observed with neutral red assay. Also, crude venom caused changes in the integrity of cell membrane by LDH release. The morphological alterations enhanced in high concentration results in total cells number reduced.
CONCLUSION
V. lebetina venom showed potential direct cytotoxic effects on human endothelial cells in a manner of concentration- dependent inhibition.
目的
血循毒毒蛇咬伤是全球严重的健康问题。蝰蛇科和蝮蛇科毒蛇咬伤后，出血是最严重的后果。人们认为血管膜降解导致出血；相反，也有人认为直接细胞毒性在内皮细胞紊乱中起作用。本研究旨在评估埃及蝰蛇粗毒对人脐静脉内皮细胞（HUVECs）的直接毒性作用。
方法
采用MTT法和中性红摄取法测定埃及蝰蛇蛇毒对HUVECs生长抑制的作用。使用细胞毒性检测试剂盒通过乳酸脱氢酶（LDH）释放来测定细胞膜的完整性。还使用相差显微镜评估内皮细胞的形态变化。
结果
在MTT试验中，粗毒对内皮细胞显示出细胞毒性作用，中性红试验观察到的结果证实了这一点。此外，粗毒通过LDH释放导致细胞膜完整性发生变化。高浓度时形态学改变增强，导致细胞总数减少。
结论
埃及蝰蛇毒液对人内皮细胞显示出潜在的直接细胞毒性作用，呈浓度依赖性抑制方式。
Zotero 插件