通过乳酸脱氢酶活性释放检测坏死。
Detection of necrosis by release of lactate dehydrogenase activity.
作者信息
Chan Francis Ka-Ming, Moriwaki Kenta, De Rosa María José
机构信息
Immunology and Virology Program, Department of Pathology, Center for AIDS Research, University of Massachusetts Medical School, Worcester, MA, USA.
出版信息
Methods Mol Biol. 2013;979:65-70. doi: 10.1007/978-1-62703-290-2_7.
Abstract
Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrosis. A key signature for necrotic cells is the permeabilization of the plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the intracellular enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is a useful method for the detection of necrosis. In this chapter, we describe the step-by-step procedure for detection of LDH release from necrotic cells using a microtiter plate-based colorimetric absorbance assay.
摘要
凋亡和坏死是在正常及疾病病理过程中观察到的两种主要细胞死亡形式。尽管有许多检测凋亡的方法，但用于测量坏死的方法相对较少。坏死细胞的一个关键特征是质膜通透性增加。通过测量细胞内酶乳酸脱氢酶（LDH）的释放，可以在组织培养环境中对这一事件进行量化。将其与其他方法结合使用时，测量LDH释放是检测坏死的一种有用方法。在本章中，我们描述了使用基于微孔板的比色吸光度测定法检测坏死细胞中LDH释放的详细步骤。
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