Larsson Anders, Carlsson Lena, Lind Anne-Li, Gordh Torsten, Bodolea Constantin, Kamali-Moghaddam Masood, Thulin Måns
Department of Medical Sciences, Uppsala University, SE-751 85 Uppsala, Sweden.
Department of Medical Sciences, Uppsala University, SE-751 85 Uppsala, Sweden.
Cytokine. 2015 Dec;76(2):514-518. doi: 10.1016/j.cyto.2015.07.010. Epub 2015 Jul 15.
Cytokines and chemokines regulate many functions in the body including the brain. The interactions between adipose tissue and the central nervous system (CNS) are important for the regulation of energy balance. CNS function is also influenced by age. The aim of the present study was to investigate the effects of body mass index (BMI) and age on cytokine and chemokine levels in cerebrospinal fluid. Cerebrospinal fluid samples (n=89) were collected from patients undergoing routine surgical procedures. The samples were analyzed using the multiplex proximity extension assay (PEA) in which 92 different cytokines are measured simultaneously using minute sample volume. We found no significant correlations between age and cytokine levels for any of the studied markers. In contrast, at a false discovery rate of 10%, 19 markers were significantly associated with BMI (in decreasing significance: FGF-5, ADA, Beta-NGF, CD40, IL-10RB, CCL19, TGF-alpha, SIRT2, TWEAK, SCF, CSF-1, 4E-BP1, DNER, LIF-R, STAMPB, CXCL10, CXCL6, VEGF-A and CX3CL1). This study reveals a clear effect of BMI on cytokine and chemokine levels in cerebrospinal fluid.
细胞因子和趋化因子调节身体包括大脑在内的许多功能。脂肪组织与中枢神经系统（CNS）之间的相互作用对于能量平衡的调节很重要。中枢神经系统功能也受年龄影响。本研究的目的是调查体重指数（BMI）和年龄对脑脊液中细胞因子和趋化因子水平的影响。从接受常规外科手术的患者中收集脑脊液样本（n = 89）。使用多重邻近延伸分析（PEA）对样本进行分析，该分析使用微量样本体积同时测量92种不同的细胞因子。我们发现，对于任何研究的标志物，年龄与细胞因子水平之间均无显著相关性。相比之下，在错误发现率为10%时，19种标志物与BMI显著相关（显著性递减：FGF-5、ADA、β-NGF、CD40、IL-10RB、CCL19、TGF-α、SIRT2、TWEAK、SCF、CSF-1、4E-BP1、DNER、LIF-R、STAMPB、CXCL10、CXCL6、VEGF-A和CX3CL1）。这项研究揭示了BMI对脑脊液中细胞因子和趋化因子水平有明显影响。
