Ishizu Takaaki, Minohara Motozumi, Ichiyama Takashi, Kira Ryutaro, Tanaka Masahito, Osoegawa Manabu, Hara Toshiro, Furukawa Susumu, Kira Jun-ichi
Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
J Neuroimmunol. 2006 Jun;175(1-2):52-8. doi: 10.1016/j.jneuroim.2006.03.020. Epub 2006 May 11.
We simultaneously measured 16 cytokines/chemokines in cerebrospinal fluid (CSF) from 14 patients with acute disseminated encephalomyelitis (ADEM) and 20 controls using a fluorescent bead-based immunoassay. A variety of cytokines, such as IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IFN-gamma, TNF-alpha, G-CSF and MIP-1beta, were significantly elevated in ADEM. In particular, G-CSF showed a marked 38-fold increase compared to the control mean. Significant positive correlations with inflammatory parameters in CSF, such as cell counts and protein levels, were found for IFN-gamma, IL-6 and IL-8. In contrast, IL-17 produced by activated CD4(+) memory T cells was not increased. The results suggested that various cytokines related to activation of macrophages/microglias and Th(1) and Th(2) cells are upregulated in CSF in ADEM.
我们使用基于荧光微珠的免疫测定法，同时检测了14例急性播散性脑脊髓炎（ADEM）患者和20名对照者脑脊液（CSF）中的16种细胞因子/趋化因子。多种细胞因子，如IL-1β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IFN-γ、TNF-α、G-CSF和MIP-1β，在ADEM中显著升高。特别是，与对照平均值相比，G-CSF显著增加了38倍。IFN-γ、IL-6和IL-8与CSF中的炎症参数（如细胞计数和蛋白质水平）呈显著正相关。相比之下，活化的CD4(+)记忆T细胞产生的IL-17没有增加。结果表明，与巨噬细胞/小胶质细胞以及Th(1)和Th(2)细胞活化相关的多种细胞因子在ADEM患者的CSF中上调。
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