水蕹对瑞士白化小鼠艾氏腹水癌的抗肿瘤活性。

Antitumor activity of Aponogeton undulatus against Ehrilich ascites carcinoma in Swiss albino mice.

作者信息

Islam Md Reyazul, Alam Md Badrul, Tamima Umme, Jenny Shayla Islam

机构信息

Department of Bioactive Materials Science, Chonbuk National University, 567, Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, Republic of Korea.

Graduate School of Food Science & Biotechnology Kyungpook National University, 80, Daehak-ro, Buk-gu, Daegu, Republic of Korea; Department of Pharmacy, AtishDipankar University of Science & Technology, Dhaka, Bangladesh.

出版信息

Asian Pac J Trop Med. 2015 Jun;8(6):431-7. doi: 10.1016/j.apjtm.2015.05.010. Epub 2015 Jun 25.

DOI:10.1016/j.apjtm.2015.05.010

PMID:26194826

Abstract

OBJECTIVE

To investigate in vitro antioxidant and in vivo antitumor activity of the crude methanolic extract of Aponogeton undulatus (A. undulatus) (MAU) along with its various organic fractions.

METHODS

A. undulatus leaves were successively extracted using methanol (MAU) and then fractionated by chloroform, ethyl acetate (EAU) and water. The total antioxidant capacity, lipid peroxidation inhibition assay, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and ferrous reducing power assessment were used to evaluate the antioxidant potential of the crude extract and its organic fractions. The in vivo antitumor activity is evaluated against Ehrlich ascites carcinoma (EAC) cell bearing in Swiss albino mice.

RESULTS

EAU showed the highest antioxidant capacity as (175.80 ± 0.41) mg/g, IC50 value of DPPH scavenging activity was (38.84 ± 0.02) μg/mL and also exhibited maximum lipid peroxidation inhibition activity with the IC50 value of (42.52 ± 0.32) μg/mL than other fractions. The results demonstrate that reducing power of the extract was concentration dependent. In addition, EAU was administered at 50, 100 and 200 mg/kg body weight respectively to EAC cell bearing mice and a significant (P < 0.05) decrease in tumor volume, packed cell volume and viable cell count and also increased the life span (17.52%, 42.53% and 62.05%). Hematological profiles were restored to normal levels in MAU treated mice as compared to EAC control mice.

CONCLUSIONS

The results were found to be significant and confirmed that the A. undulatus has remarkable antitumor activity with antioxidant potential.

摘要

目的

研究波叶海菜花甲醇粗提物（MAU）及其不同有机相组分的体外抗氧化活性和体内抗肿瘤活性。

方法

采用甲醇对波叶海菜花叶片进行提取得到MAU，然后依次用氯仿、乙酸乙酯（EAU）和水对其进行分离。通过总抗氧化能力、脂质过氧化抑制试验、1,1-二苯基-2-苦基肼（DPPH）自由基清除试验和亚铁还原能力评估，来评价粗提物及其有机相组分的抗氧化潜力。以瑞士白化小鼠体内接种艾氏腹水癌（EAC）细胞来评估其体内抗肿瘤活性。

结果

EAU表现出最高的抗氧化能力，为（175.80±0.41）mg/g，DPPH清除活性的IC50值为（38.84±0.02）μg/mL，并且与其他组分相比，还表现出最大的脂质过氧化抑制活性，IC50值为（42.52±0.32）μg/mL。结果表明提取物的还原能力呈浓度依赖性。此外，分别以50、100和200mg/kg体重的剂量给接种EAC细胞的小鼠施用EAU，肿瘤体积、红细胞压积和活细胞计数显著（P<0.05）降低，同时还延长了小鼠寿命（分别为17.52%、42.53%和62.05%）。与EAC对照小鼠相比，MAU处理的小鼠血液学指标恢复到正常水平。