Alam Badrul, Majumder Rajib, Akter Shahina, Lee Sang-Han
Department of Food Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea ; Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka 1213, Bangladesh.
Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka 1213, Bangladesh.
Oncol Lett. 2015 Feb;9(2):863-868. doi: 10.3892/ol.2014.2738. Epub 2014 Nov 25.
The present study was conducted to evaluate the methanolic extract of leaves (MPBL) and its organic fractions with regard to antitumor activity against Ehrlich ascites carcinoma (EAC) in Swiss albino mice and to confirm their antioxidant activities. At 24 h post-intraperitoneal inoculation of tumor cells into mice, extracts were administered at 25, 50 and 100 mg/kg body weight for nine consecutive days. The antitumor effects of the extracts were then assessed according to tumor volume, packed cell count, viable and non-viable tumor cell count, median survival time and increase in life span of EAC-bearing mice. Next, hematological profiles and serum biochemical parameters were calculated, and antioxidant properties were assessed by estimating lipid peroxidation, reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) levels. MPBL and the ethylacetate fraction (EPBL) at a dose of 100 mg/kg induced a significant decrease in tumor volume, packed cell volume and viable cell count and increased the life span of the EAC-bearing mice (P<0.05). Hematological and serum biochemical profiles were restored to normal levels in the extract-treated mice compared with the EAC control mice. MPBL and EPBL treatment significantly decreased lipid peroxidation (P<0.05) and restored GSH, SOD and CAT levels towards normal compared with the EAC control. Taken together, the results of the present study demonstrated that extracts exhibit significant antitumor activity, which may be attributed to the augmentation of endogenous antioxidant potential.
本研究旨在评估叶的甲醇提取物（MPBL）及其有机馏分对瑞士白化小鼠艾氏腹水癌（EAC）的抗肿瘤活性，并确认其抗氧化活性。在给小鼠腹腔接种肿瘤细胞24小时后，以25、50和100毫克/千克体重的剂量连续九天给予提取物。然后根据肿瘤体积、血细胞压积、活肿瘤细胞和非活肿瘤细胞计数、中位生存时间以及荷EAC小鼠的寿命延长情况评估提取物的抗肿瘤效果。接下来，计算血液学指标和血清生化参数，并通过估计脂质过氧化、还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平来评估抗氧化特性。剂量为100毫克/千克的MPBL和乙酸乙酯馏分（EPBL）可使肿瘤体积、血细胞压积和活细胞计数显著降低，并延长荷EAC小鼠的寿命（P<0.05）。与EAC对照小鼠相比，提取物处理的小鼠的血液学和血清生化指标恢复到正常水平。与EAC对照相比，MPBL和EPBL处理可显著降低脂质过氧化（P<0.05），并使GSH、SOD和CAT水平恢复正常。综上所述，本研究结果表明，提取物具有显著的抗肿瘤活性，这可能归因于内源性抗氧化潜力的增强。
