Gupta Malaya, Mazumder Upal Kanti, Kumar Ramanathan Sambath, Sivakumar Thangavel, Vamsi Madgula Lakshmi Mohan
Division of Pharmacology and Pharmaceutical Chemistry, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.
J Pharmacol Sci. 2004 Feb;94(2):177-84. doi: 10.1254/jphs.94.177.
The methanol extract of Caesalpinia bonducella FLEMING (Caesalpiniaceae) leaves (MECB) were evaluated for antitumor activity against Ehrlich ascites carcinoma (EAC)-bearing Swiss albino mice. The extract was administered at the doses of 50, 100, and 200 mg/kg body weight per day for 14 days after 24 h of tumor inoculation. After the last dose and 18 h fasting, the mice were sacrificed. The present study deals with the effect of MECB on the growth of transplantable murine tumor, life span of EAC-bearing hosts, hematological profile, and biochemical parameters such as lipid peroxidation (LPO), glutathione content (GSH), superoxide dismutase (SOD), and catalase (CAT) activities. MECB caused significant (P<0.01) decrease in tumor volume, packed cell volume, and viable cell count; and it prolonged the life span of EAC-tumor bearing mice. Hematological profile converted to more or less normal levels in extract-treated mice. MECB significantly (P<0.05) decreased the levels of lipid peroxidation and significantly (P<0.05) increased the levels of GSH, SOD, and CAT. The MECB was found to be devoid of conspicuous short-term toxicity in the mice when administered daily (i.p.) for 14 days at the doses of 50, 100, 200, and 300 mg/kg. The treated mice showed conspicuous toxic symptoms only at 300 mg/kg. The results indicate that MECB exhibited significant antitumor and antioxidant activity in EAC-bearing mice.
对决明属植物（苏木科）叶的甲醇提取物（MECB）针对携带艾氏腹水癌（EAC）的瑞士白化小鼠的抗肿瘤活性进行了评估。在接种肿瘤24小时后，以每天50、100和200毫克/千克体重的剂量给予提取物，持续14天。在最后一剂给药后并禁食18小时后，处死小鼠。本研究探讨了MECB对可移植性小鼠肿瘤生长、携带EAC宿主的寿命、血液学指标以及脂质过氧化（LPO）、谷胱甘肽含量（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性等生化参数的影响。MECB使肿瘤体积、红细胞压积和活细胞计数显著（P<0.01）降低；并延长了携带EAC肿瘤小鼠的寿命。提取物处理的小鼠的血液学指标或多或少恢复到正常水平。MECB显著（P<0.05）降低了脂质过氧化水平，并显著（P<0.05）提高了GSH、SOD和CAT的水平。当以50、100、200和300毫克/千克的剂量每天（腹腔注射）给药14天时，发现MECB在小鼠中没有明显的短期毒性。仅在300毫克/千克时，处理过的小鼠出现明显的毒性症状。结果表明，MECB在携带EAC的小鼠中表现出显著的抗肿瘤和抗氧化活性。
