Chen Wen-Ming, Chen Jhen-Ci, Wang Chi, Huang Cheng-Wen, Sheu Shih-Yi
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2015 Oct;65(10):3634-3640. doi: 10.1099/ijsem.0.000467.
A bacterial strain, designated Npb-02T, was isolated from a freshwater river in Taiwan and characterized in a taxonomic study using a polyphasic approach. Cells of strain Npb-02T were Gram-stain-negative, aerobic, poly-β-hydroxybutyrate-accumulating, rod-shaped and non-motile. Growth occurred at 15–40 °C (optimum 25–30 °C), at pH 7.0–8.0 (optimum pH 7.0) and with 0–1 % NaCl (optimum 0.5 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Npb-02T belonged to the genus Vogesella and was most closely related to Vogesella perlucida DS-28T with sequence similarity of 98.3 %. Strain Npb-02T contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0 as the major fatty acids. The major respiratory quinone was Q-8.The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminophospholipid and an uncharacterized phospholipid. The genomic DNA G+C content of strain Npb-02T was 64.1 mol%. The DNA–DNA hybridization values for strain Npb-02T with Vogesella perlucida DS-28T, Vogesella mureinivorans 389T and Vogesella lacus GR13T were less than 25 %. On the basis of phylogenetic inference and phenotypic data, strain Npb-02T represents a novel species of the genus Vogesella, for which the name Vogesella amnigena sp. nov. is proposed. The type strain is Npb-02T ( = BCRC 80887T = LMG 28419T = KCTC 42195T).
从台湾的一条淡水河中分离出一株细菌，命名为Npb-02T，并采用多相分类法对其进行分类学研究。Npb-02T菌株的细胞革兰氏染色阴性，好氧，能积累聚-β-羟基丁酸，杆状，无运动性。在15-40℃（最适温度25-30℃）、pH 7.0-8.0（最适pH 7.0）和0-1% NaCl（最适0.5%）条件下生长。基于16S rRNA基因序列的系统发育分析表明，Npb-02T菌株属于沃氏菌属，与透明沃氏菌DS-28T关系最密切，序列相似性为98.3%。Npb-02T菌株以特征性总和3（包括C16 : 1ω7c和/或C16 : 1ω6c）和C16 : 0作为主要脂肪酸。主要呼吸醌为Q-8。极性脂质谱由磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种未鉴定的氨基磷脂和一种未鉴定的磷脂组成。Npb-02T菌株的基因组DNA G+C含量为64.1 mol%。Npb-02T菌株与透明沃氏菌DS-28T、食壁沃氏菌389T和湖泊沃氏菌GR13T的DNA-DNA杂交值均低于25%。基于系统发育推断和表型数据，Npb-02T菌株代表沃氏菌属的一个新物种，为此提出新名称水栖沃氏菌（Vogesella amnigena sp. nov.）。模式菌株为Npb-02T（= BCRC 80887T = LMG 28419T = KCTC 42195T）。
