Sorlózano Antonio, Panesso Diana, Navarro-Marí José María, Arias Cesar A, Gutiérrez-Fernández José
José Gutiérrez-Fernández, Department of Microbiology, University of Granada Medical School, Av. de Madrid, 11 , 18012 Granada, Spain.
Rev Esp Quimioter. 2015 Aug;28(4):207-9.
Characterization of a urine isolate of daptomycin non-susceptible Enterococcus faecium recovered from a patient with kidney transplantation and no history of daptomycin exposure.
After isolation in a urine sample, identification of E. faecium was confirmed by amplification of the E. faecium-specific gene encoding D-alanyl-D-alanine ligase (ddl) and daptomycin susceptibility testing was performed by E-test on cation-adjusted Mueller-Hinton agar. In order to determine the genetic bases of daptomycin resistance, the open reading frames of five genes previously associated with daptomycin resistance in enterococci were sequenced.
Substitutions in the response regulator LiaR (S19F) and cardiolipin synthase (R218Q) were identified.
To the best of our knowledge, this is the first characterization of emerging daptomycin resistance in E. faecium in a Spanish hospital in the absence of daptomycin exposure and in a renal transplant recipient.
对从一名肾移植患者尿液中分离出的耐达托霉素屎肠球菌进行特征分析，该患者无达托霉素暴露史。
从尿液样本中分离出屎肠球菌后，通过扩增编码D -丙氨酰 - D -丙氨酸连接酶（ddl）的屎肠球菌特异性基因来确认其身份，并在阳离子调整的穆勒 - 欣顿琼脂上通过E试验进行达托霉素敏感性测试。为了确定达托霉素耐药的遗传基础，对先前与肠球菌中达托霉素耐药相关的五个基因的开放阅读框进行了测序。
在反应调节因子LiaR（S19F）和心磷脂合酶（R218Q）中发现了替代突变。
据我们所知，这是西班牙一家医院中在无达托霉素暴露情况下且在肾移植受者中首次对屎肠球菌新出现的达托霉素耐药性进行的特征分析。
