Songür Murat, Şahin Ercan, Demir Tuğcan, Kalem Mahmut, Take Kaplanoğlu Gülnur, Altun Necdet Şükrü
Bülent Ecevit University Faculty of Medicine, Department of Orthopaedics and Traumatology, Zonguldak, Turkey.
Bahçeşehir University, Medical Park Hospital, Department of Orthopaedics and Traumatology, İstanbul, Turkey.
Acta Orthop Traumatol Turc. 2015;49(3):311-8. doi: 10.3944/AOTT.2015.14.0344.
Bone protein extract (BPE) usually requires a carrier or a scaffold for implantation. We aimed to compare the effect of equine-derived BPE, an osteoinductive agent composed of a high amount of type-I collagen and other bone proteins (Colloss-E), with that of human demineralized bone matrix (DBM) for treating cavitary bone defects not requiring scaffold use.
Rabbit distal femoral condyle was used as a stable cavitary bone defect model. Bone defects of 6-mm diameter and 10-12-mm depth were created in the femoral condyles. Rabbits were assigned into the equine-derived BPE (BPE), human-derived DBM (DBM), and control (C) groups. Approximately 20 mg of BPE was implanted into the defect in the equine-derived BPE group (n=6), whereas 0.3 cc of DBM was implanted in the DBM group (n=6). Defects were left empty in the C group (n=6). The defect area was histologically examined after 6 weeks.
There were no instances of macroscopic defect collapse or failure. Histopathological examination revealed that the BPE group had better scores (statistically significant) than both the other groups in terms of quality of union. The BPE group also had higher scores than the DBM group in terms of graft incorporation and new-bone formation.
The current study revealed results consistent with those of the previous studies concerning BPEs. Equine-derived BPE was found to be successful for treating cavitary bone defects not requiring scaffold use.
骨蛋白提取物（BPE）通常需要载体或支架进行植入。我们旨在比较马源BPE（一种由大量I型胶原蛋白和其他骨蛋白组成的骨诱导剂，Colloss-E）与人类脱矿骨基质（DBM）治疗无需使用支架的空洞性骨缺损的效果。
兔股骨远端髁用作稳定的空洞性骨缺损模型。在股骨髁上制造直径6毫米、深度10 - 12毫米的骨缺损。将兔子分为马源BPE（BPE）组、人源DBM（DBM）组和对照组（C）。在马源BPE组（n = 6）的缺损处植入约20毫克BPE，而在DBM组（n = 6）植入0.3立方厘米DBM。C组（n = 6）的缺损处不植入任何物质。6周后对缺损区域进行组织学检查。
未出现宏观缺损塌陷或失败的情况。组织病理学检查显示，在愈合质量方面，BPE组的评分优于其他两组（具有统计学意义）。在移植物整合和新骨形成方面，BPE组的评分也高于DBM组。
本研究结果与先前关于BPE的研究结果一致。发现马源BPE成功用于治疗无需使用支架的空洞性骨缺损。
