Rabie A B, Wong R W, Hägg U
Orthodontics, Faculty of Dentistry, University of Hong Kong, Prince Philip Hospital, Hong Kong.
Br J Oral Maxillofac Surg. 2000 Oct;38(5):565-70. doi: 10.1054/bjom.2000.0464.
We compared the amount of new bone produced by endochondral and intramembranous autogenous bone grafts in the presence of demineralized bone matrices (DBMs) prepared from intramembranous bone (DBM(IM)) or endochondral bone (DBM(EC)). Thirty-five bone defects were created in the parietal bone of 20 New Zealand White rabbits. In the experimental groups, 5 defects were grafted with endochondral bone, 5 with endochondral bone mixed with DBM(IM)) (EC-DBM(IM)), 5 with intramembranous bone mixed with DBM(IM)(IM-DBM(IM)) and 6 with endochondral bone mixed with DBM(EC)(EC-DBM(EC)). In the control groups, 10 defects were left alone (passive control) and 4 were grafted with rabbit skin collagen (active control). They were all killed on day 14 and the defects were prepared for histological study. Serial sections were cut across the whole defect. Quantitative analyses were made on 202 sections of the experimental groups by image analysis. A total of 414%, 708%, and 85% more new bone was formed in defects grafted with composite EC-DBM(IM), IM-DBM(IM)and EC-DBM(EC), respectively, than those grafted with endochondral bone alone (P<0.001). No bone was formed in either passive or active controls. In conclusion, demineralized bone matrices, particularly those derived from intramembranous bone, have extremely high osteoinductive properties and greatly improve the integration of autogenous bone grafts in the skull.
我们比较了软骨内和膜内自体骨移植在存在由膜内骨（DBM(IM)）或软骨内骨（DBM(EC)）制备的脱矿骨基质（DBM）时产生的新骨量。在20只新西兰白兔的顶骨上制造了35个骨缺损。在实验组中，5个缺损用软骨内骨移植，5个用软骨内骨与DBM(IM)混合（EC-DBM(IM)），5个用膜内骨与DBM(IM)混合（IM-DBM(IM)），6个用软骨内骨与DBM(EC)混合（EC-DBM(EC)）。在对照组中，10个缺损不做处理（被动对照），4个用兔皮胶原移植（主动对照）。它们在第14天全部处死，对缺损进行组织学研究。在整个缺损处切取连续切片。通过图像分析对实验组的202个切片进行定量分析。与单独用软骨内骨移植的缺损相比，分别用复合EC-DBM(IM)、IM-DBM(IM)和EC-DBM(EC)移植的缺损中形成的新骨分别多414%、708%和85%（P<0.001）。被动或主动对照组均未形成骨。总之，脱矿骨基质，特别是那些源自膜内骨的脱矿骨基质，具有极高的骨诱导特性，并大大改善了自体骨移植在颅骨中的整合。
