Da-Silva C F, Langone F
Departamento de Anatomia, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 1989;22(6):691-4.
The sciatic nerve of adult mice was transected and the proximal and distal nerve stumps were sutured into a polyethylene tube. The tubes were implanted either empty, or the lumen was filled with pure collagen or a mixture of collagen/nerve growth factor (NGF). Six weeks later, cells in the L3-L5 dorsal root ganglia (DRG) were retrogradely filled with horseradish peroxidase (HRP). The data demonstrate that the addition of NGF to the interior of the tubular prosthesis can significantly increase the regeneration rate of sensory neurons.
将成年小鼠的坐骨神经切断，将近端和远端神经残端缝合到一根聚乙烯管中。这些管子要么空着植入，要么管腔内填充纯胶原蛋白或胶原蛋白/神经生长因子（NGF）的混合物。六周后，用辣根过氧化物酶（HRP）对L3-L5背根神经节（DRG）中的细胞进行逆行填充。数据表明，在管状假体内部添加NGF可显著提高感觉神经元的再生率。
