Madison R, da Silva C F, Dikkes P, Chiu T H, Sidman R L
Exp Neurol. 1985 Jun;88(3):767-72. doi: 10.1016/0014-4886(85)90087-1.
The sciatic nerve of adult mice was transected and proximal and distal nerve stumps were sutured into a nontoxic bioresorbable nerve guide. Nerve guide lumens were either empty or filled with a gel containing 80% laminin and additional extracellular matrix components. Two weeks later cells in the L3 through L5 dorsal root ganglia and the ventral horn of the spinal cord were retrogradely filled with horseradish peroxidase. All animals with the laminin-containing gel but none with empty nerve guides displayed labeled cells. This suggests that the laminin-containing gel significantly hastened axonal regeneration in vivo.
将成年小鼠的坐骨神经切断，把近端和远端神经残端缝合到一个无毒的生物可吸收神经导管中。神经导管管腔要么是空的，要么填充有含有80%层粘连蛋白和其他细胞外基质成分的凝胶。两周后，用辣根过氧化物酶对L3至L5背根神经节和脊髓腹角中的细胞进行逆行填充。所有含有层粘连蛋白凝胶的动物都显示有标记细胞，而神经导管为空的动物则没有。这表明含层粘连蛋白的凝胶在体内显著加速了轴突再生。
