Oehmichen M, Linke P, Zilles K, Saternus K S
Institut für Rechtsmedizin, Universität Köln, FRG.
Clin Neuropathol. 1989 Nov-Dec;8(6):276-83.
The investigation was carried out on 11 sudden infant death (SIDS) cases which were compared with age- and sex-matched controls. Six brain nuclei were selected for evaluation. Using immunohistochemical methods, macrophages were selectively demonstrated by detection of lysozyme; reactive astrocytes, by detection of intracytoplasmic albumin (marker of prior impairment of blood-brain barrier function) or GFAP. No lysozyme-positive cells were demonstrable in the brain stem of any of the examined cases. Although a greater number of reactive, GFAP-expressing astrocytes were found in the SIDS cases, 3 of the 11 SIDS cases (compared to 5 controls) had no reactive astrocytes in any of examined brain nuclei. Reactive astrocytes, however, were identified in more than half the controls. Sections treated with anti-albumin serum were evaluated quantitatively. Total number of non-neuronal cells, relative proportion of astrocytes, and proportion of albumin-positive astrocytes were determined. Paired brain nuclei were counted on both sides of the brain stem. The number of non-neuronal cells, astrocytes, and albumin-positive astrocytes in the SIDS cases did not differ significantly from those in the controls. No statistically relevant difference was established between the right and left parts of the brain stem. The findings were discussed in light of the literature.
对11例婴儿猝死（SIDS）病例进行了调查，并与年龄和性别匹配的对照组进行比较。选择了六个脑核进行评估。采用免疫组织化学方法，通过检测溶菌酶选择性显示巨噬细胞；通过检测细胞质内白蛋白（血脑屏障功能先前受损的标志物）或胶质纤维酸性蛋白（GFAP）显示反应性星形胶质细胞。在所检查的任何病例的脑干中均未发现溶菌酶阳性细胞。尽管在SIDS病例中发现了更多表达GFAP的反应性星形胶质细胞，但11例SIDS病例中有3例（与5例对照相比）在所检查的任何脑核中均无反应性星形胶质细胞。然而，在超过一半的对照组中发现了反应性星形胶质细胞。对用抗白蛋白血清处理的切片进行定量评估。确定非神经元细胞的总数、星形胶质细胞的相对比例以及白蛋白阳性星形胶质细胞的比例。对脑干两侧的配对脑核进行计数。SIDS病例中非神经元细胞、星形胶质细胞和白蛋白阳性星形胶质细胞的数量与对照组相比无显著差异。脑干左右部分之间未发现统计学上的相关差异。结合文献对研究结果进行了讨论。
