Safaei Zahra, Karimi Keikhosro, Golkar Poorandokht, Zamani Akram
Department of Chemical Engineering, Isfahan University of Technology, 84156-83111 Isfahan, Iran.
Institutes of Biotechnology and Bioengineering, Isfahan University of Technology, 84156-83111 Isfahan, Iran.
Int J Mol Sci. 2015 Jul 22;16(7):16683-94. doi: 10.3390/ijms160716683.
The objective of this study was to investigate the effects of indole-3-acetic acid (IAA) and kinetin (KIN) on Mucor indicus growth, cell wall composition, and ethanol production. A semi-synthetic medium, supplemented with 0-5 mg/L hormones, was used for the cultivations (at 32 °C for 48 h). By addition of 1 mg/L of each hormone, the biomass and ethanol yields were increased and decreased, respectively. At higher levels, however, an inverse trend was observed. The glucosamine fraction of the cell wall, as a representative for chitosan, followed similar but sharper changes, compared to the biomass. The highest level was 221% higher than that obtained without hormones. The sum of glucosamine and N-acetyl glucosamine (chitin and chitosan) was noticeably enhanced in the presence of the hormones. Increase of chitosan was accompanied by a decrease in the phosphate content, with the lowest phosphate (0.01 g/g cell wall) being obtained when the chitosan was at the maximum (0.45 g/g cell wall). In conclusion, IAA and KIN significantly enhanced the M. indicus growth and chitosan production, while at the same time decreasing the ethanol yield to some extent. This study shows that plant growth hormones have a high potential for the improvement of fungal chitosan production by M. indicus.
本研究的目的是调查吲哚 - 3 - 乙酸（IAA）和激动素（KIN）对印度毛霉生长、细胞壁组成及乙醇产量的影响。使用添加了0 - 5 mg/L激素的半合成培养基进行培养（在32°C下培养48小时）。添加1 mg/L的每种激素后，生物量和乙醇产量分别增加和降低。然而，在更高水平时，观察到相反的趋势。作为壳聚糖代表的细胞壁中氨基葡萄糖部分，与生物量相比，呈现出相似但更明显的变化。最高水平比未添加激素时高出221%。在激素存在的情况下，氨基葡萄糖和N - 乙酰氨基葡萄糖（几丁质和壳聚糖）的总和显著增加。壳聚糖的增加伴随着磷酸盐含量的降低，当壳聚糖达到最大值（0.45 g/g细胞壁）时，磷酸盐含量最低（0.01 g/g细胞壁）。总之，IAA和KIN显著促进了印度毛霉的生长和壳聚糖的产生，同时在一定程度上降低了乙醇产量。本研究表明，植物生长激素在提高印度毛霉真菌壳聚糖产量方面具有很大潜力。
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