采用质谱法对脑脊液中淀粉样蛋白-β 1-42 进行定量的轮次测试。
Round robin test on quantification of amyloid-β 1-42 in cerebrospinal fluid by mass spectrometry.
机构信息
Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Salgrenska Academy, University of Gothenburg, Mölndal, Sweden.
Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Salgrenska Academy, University of Gothenburg, Mölndal, Sweden.
出版信息
Alzheimers Dement. 2016 Jan;12(1):55-9. doi: 10.1016/j.jalz.2015.06.1890. Epub 2015 Jul 21.
INTRODUCTION
Cerebrospinal fluid (CSF) amyloid-β 1-42 (Aβ42) is an important biomarker for Alzheimer's disease, both in diagnostics and to monitor disease-modifying therapies. However, there is a great need for standardization of methods used for quantification. To overcome problems associated with immunoassays, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has emerged as a critical orthogonal alternative.
METHODS
We compared results for CSF Aβ42 quantification in a round robin study performed in four laboratories using similar sample preparation methods and LC-MS instrumentation.
RESULTS
The LC-MS results showed excellent correlation between laboratories (r(2) >0.98), high analytical precision, and good correlation with enzyme-linked immunosorbent assay (r(2) >0.85). The use of a common reference sample further decreased interlaboratory variation.
DISCUSSION
Our results indicate that LC-MS is suitable for absolute quantification of Aβ42 in CSF and highlight the importance of developing a certified reference material.
简介
脑脊液（CSF）中的淀粉样蛋白-β 1-42（Aβ42）是阿尔茨海默病的一个重要生物标志物，无论是在诊断方面还是在监测疾病修饰疗法方面都是如此。然而，对于用于定量的方法，标准化的需求非常大。为了克服免疫测定相关的问题，液相色谱-串联质谱（LC-MS/MS）已成为一种重要的正交替代方法。
方法
我们比较了在四个实验室中使用类似的样品制备方法和 LC-MS 仪器进行的一项轮次间研究中 CSF Aβ42 定量的结果。
结果
LC-MS 结果显示实验室之间具有出色的相关性（r(2) >0.98），具有较高的分析精密度，并且与酶联免疫吸附测定（ELISA）具有良好的相关性（r(2) >0.85）。使用共同的参考样本进一步降低了实验室间的差异。
讨论
我们的结果表明 LC-MS 适用于 CSF 中 Aβ42 的绝对定量，并强调了开发经过认证的参考物质的重要性。
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