Willemse Eline A J, Tijms Betty M, van Berckel Bart N M, Le Bastard Nathalie, van der Flier Wiesje M, Scheltens Philip, Teunissen Charlotte E
Department of Clinical Chemistry Neurochemistry Laboratory Amsterdam Neuroscience Vrije Universiteit Amsterdam Amsterdam UMC Amsterdam the Netherlands.
Department of Neurology Alzheimer Center Amsterdam Neuroscience Vrije Universiteit Amsterdam Amsterdam UMC Amsterdam the Netherlands.
Alzheimers Dement (Amst). 2021 May 1;13(1):e12182. doi: 10.1002/dad2.12182. eCollection 2021.
We evaluated for two novel automated biomarker assays how cerebrospinal fluid (CSF) amyloid beta (Aβ)-ratios improved the concordance with amyloid positron emission tomography (PET) positivity compared to Aβ alone.
We selected 288 individuals from the Amsterdam Dementia Cohort across the Alzheimer's disease clinical spectrum when they had both CSF and amyloid PET visual read available, regardless of diagnosis. CSF Aβ, phosphorylated tau (p-tau), and total tau (t-tau) were measured with Elecsys and Lumipulse assays, and Aβ with Lumipulse. CSF cut-points were defined using receiver operating characteristic (ROC) for amyloid PET positivity.
For both Elecsys and Lumipulse the p-tau/Aβ, Aβ/Aβ, and t-tau/Aβ ratios showed similarly good concordance with amyloid PET (Elecsys: 93,90,90%; Lumipulse: 94,92,90%) and were higher than Aβ alone (Elecsys 85%; Lumipulse 84%).
Biomarker ratios p-tau/Aβ, Aβ/Aβ, t-tau/Aβ on two automated platforms show similar optimal concordance with amyloid PET in a memory clinic cohort.
我们评估了两种新型自动化生物标志物检测方法，与单独检测淀粉样蛋白β（Aβ）相比，脑脊液（CSF）淀粉样蛋白β比值如何提高与淀粉样蛋白正电子发射断层扫描（PET）阳性结果的一致性。
我们从阿姆斯特丹痴呆队列中选取了288名处于阿尔茨海默病临床谱系的个体，这些个体同时具备脑脊液和淀粉样蛋白PET视觉读数，无论其诊断结果如何。使用罗氏电化学发光免疫分析法（Elecsys）和日本东曹公司的Lumipulse检测法测量脑脊液中的Aβ、磷酸化tau蛋白（p-tau）和总tau蛋白（t-tau），并使用Lumipulse检测法测量Aβ。通过接受者操作特征（ROC）曲线确定淀粉样蛋白PET阳性的脑脊液切点。
对于Elecsys和Lumipulse检测法，p-tau/Aβ、Aβ/Aβ和t-tau/Aβ比值与淀粉样蛋白PET的一致性同样良好（Elecsys：93%、90%、90%；Lumipulse：94%、92%、90%），且高于单独检测Aβ（Elecsys为85%；Lumipulse为84%）。
在记忆门诊队列中，两个自动化平台上的生物标志物比值p-tau/Aβ、Aβ/Aβ、t-tau/Aβ与淀粉样蛋白PET显示出相似的最佳一致性。
