Nagamatsu Y, Okamoto U, Yamamoto J, Tsuda Y, Okada Y
Faculty of Nutrition, Kobe-Gakuin University, Japan.
Haemostasis. 1989;19(6):322-30. doi: 10.1159/000216078.
Nonplasmin-mediated fibrinolytic activities were extracted with 2 M NaCIO4 from the spleen and leukocytes of nine species of mammals. The amidolytic and fibrinolytic activities of extracts were measured using Suc-Ala-Tyr-Leu-Val-pNA for elastase-like protease (ELP) and Suc-Tyr-Leu-Phe-pNA (or Suc-Ile-Pro-Phe-pNA) for chymotrypsin-like protease (CLP). ELP and the fibrinolytic activity in the human spleen and leukocytes exhibited the highest values among the nine species. The relative order of amidolysis by CLP was as follows: hamster greater than rat greater than mouse greater than dog in the spleen and hamster greater than rat = ox greater than dog in leukocytes. Neither fibrinolytic nor amidolytic activity was measurable in spleens and leukocytes of pigs and rabbits. The ratios of ELP to CLP activity in the spleen or leukocytes of each of the species varied significantly from those observed in humans. The amidolytic activity of the spleen in control mice was low; however, that of mice which had been intraperitoneally inoculated with sarcoma 180 cells was enhanced and further accompanied by an increase in the number of granulocytes and in fibrinolytic activity. These results suggest that the enhancement of spleen amidolytic and fibrinolytic activities is induced by an increase of granulocytes in the circulation.
用2M高氯酸钠从9种哺乳动物的脾脏和白细胞中提取非纤溶酶介导的纤溶活性。提取物的酰胺水解活性和纤溶活性分别使用用于弹性蛋白酶样蛋白酶（ELP）的Suc-Ala-Tyr-Leu-Val-pNA和用于胰凝乳蛋白酶样蛋白酶（CLP）的Suc-Tyr-Leu-Phe-pNA（或Suc-Ile-Pro-Phe-pNA）进行测定。在这9种动物中，人脾脏和白细胞中的ELP及纤溶活性最高。CLP酰胺水解的相对顺序如下：在脾脏中，仓鼠＞大鼠＞小鼠＞狗；在白细胞中，仓鼠＞大鼠 = 牛＞狗。猪和兔的脾脏及白细胞中均未检测到纤溶活性和酰胺水解活性。各物种脾脏或白细胞中ELP与CLP活性的比值与人类显著不同。对照小鼠脾脏的酰胺水解活性较低；然而，腹腔接种肉瘤180细胞的小鼠，其酰胺水解活性增强，同时粒细胞数量增加，纤溶活性也随之增加。这些结果表明，循环中粒细胞数量增加可诱导脾脏酰胺水解活性和纤溶活性增强。
