Nagamatsu Y, Yamamoto J, Fukuda A, Ohta M, Tsuda Y, Okada Y
Faculty of Nutrition, Kobe-Gakuin University, Japan.
Haemostasis. 1991;21(6):338-45. doi: 10.1159/000216247.
The concentration of leukocyte elastase (ELP) in plasma and serum was determined by an amidolysis method using a specific synthetic substrate for ELP, Suc-Ala-Tyr-Leu-Val-pNA. Results were compared with those using ELISA. ELP levels in plasma from healthy donors were similar to those determined by ELISA; however, the levels in serum were lower than those determined by ELISA. Correlation coefficients of ELP levels in plasma and serum as measured by the two methods were 0.75 (amidolysis) and 0.90 (ELISA). On the other hand, the correlation coefficient between serum ELP by the two methods was 0.83. Half of the ELP levels in plasma from 150 patients and serum from 400 patients were significantly elevated when compared with those from healthy donors, and the ELP elevation determined by amidolytic assay correlated with some variables in blood, namely fibrin(ogen)-degraded products, fibrinogen, GOT, GPT, gamma-GTP, LDH and leucine aminopeptidase. Despite the fact that the amidolysis method detects the alpha 2-macroglobulin-ELP complex while ELISA detects the alpha 1-antitrypsin-ELP complex, a comparative study showed amidolysis to provide sufficiently sensitive measurement of both plasma and serum ELP.
采用一种针对白细胞弹性蛋白酶（ELP）的特异性合成底物Suc-Ala-Tyr-Leu-Val-pNA的酰胺水解法，测定血浆和血清中白细胞弹性蛋白酶（ELP）的浓度。将结果与采用酶联免疫吸附测定法（ELISA）的结果进行比较。健康供体血浆中的ELP水平与ELISA法测定的结果相似；然而，血清中的水平低于ELISA法测定的结果。两种方法测定的血浆和血清中ELP水平的相关系数分别为0.75（酰胺水解法）和0.90（ELISA法）。另一方面，两种方法测定的血清ELP之间的相关系数为0.83。与健康供体相比，150例患者血浆和400例患者血清中一半的ELP水平显著升高，并且通过酰胺水解测定法确定的ELP升高与血液中的一些变量相关，即纤维蛋白（原）降解产物、纤维蛋白原、谷草转氨酶、谷丙转氨酶、γ-谷氨酰转肽酶、乳酸脱氢酶和亮氨酸氨肽酶。尽管酰胺水解法检测的是α2-巨球蛋白-ELP复合物，而ELISA法检测的是α1-抗胰蛋白酶-ELP复合物，但一项比较研究表明，酰胺水解法能够对血浆和血清ELP进行足够灵敏的测量。
