Rêgo Moacyr Jesus Barreto Melo, da Silva Rafael Ramos, Pereira Michelly Cristiny, da Silva Araújo Aderson, Pitta Ivan da Rocha, Falcão Diego Arruda, Bezerra Marcos André Cavalcanti, Pitta Maira Galdino da Rocha
Laboratory of Immunomodulation and Novel Therapeutic Approaches (LINAT), Research Center for Therapeutic Innovation (NUPIT), Universidade Federal de Pernambuco (UFPE), Recife 50670-901, Brazil.
Hematology and Hemotherapy Foundation of Pernambuco (HEMOPE), Recife 52011-900, Brazil.
Cytokine. 2015 Oct;75(2):310-5. doi: 10.1016/j.cyto.2015.07.016. Epub 2015 Jul 22.
Leg ulcers (LUs) are a debilitating complication of sickle cell anemia (SCA), with inflammation known to play a crucial role in their pathogenesis. Many studies have described the roles of T helper type 1 (Th1) and Th2 pathways in SCA; however, defects in anti-inflammatory responses are poorly understood. We evaluated interleukin (IL)-10 levels in serum and peripheral blood mononuclear cells (PBMCs) in SCA patients with leg ulcers (SCALU) and without leg ulcers (SCAWH) in addition to CD4(+) CD25(+)FoxP3(+) T cell populations and their its IL-10 expression. In stimulated and unstimulated PBMC cultures, SCALU patients produced higher levels of IL-10 than those in the SCAWH group. Higher levels of IL-10 in SCALU patients correlated with a history of osteonecrosis in stimulated and unstimulated cultures when compared with those in SCAWH. Immunophenotyping revealed that SCALU patients had a higher proportion of CD4(+)CD25(+)FoxP3(+), Tr1 and CD4(+)CD25(+)FoxP3(+)IL-10(+) T cells than other groups. Our findings revealed that IL-10 levels were increased in unstimulated cells from the SCALU group, and that this group also presented with a predominant CD4(+) CD25(+)FoxP3(+) cell population despite many of those cells being IL-10 negative.
腿部溃疡（LUs）是镰状细胞贫血（SCA）的一种使人衰弱的并发症，已知炎症在其发病机制中起关键作用。许多研究描述了1型辅助性T细胞（Th1）和Th2途径在SCA中的作用；然而，对抗炎反应缺陷的了解却很少。我们评估了患有腿部溃疡的SCA患者（SCALU）和未患腿部溃疡的SCA患者（SCAWH）血清和外周血单核细胞（PBMCs）中的白细胞介素（IL）-10水平，以及CD4(+) CD25(+)FoxP3(+) T细胞群体及其IL-10表达。在刺激和未刺激的PBMC培养物中，SCALU患者产生的IL-10水平高于SCAWH组。与SCAWH组相比，SCALU患者在刺激和未刺激培养物中较高的IL-10水平与骨坏死病史相关。免疫表型分析显示，SCALU患者的CD4(+)CD25(+)FoxP3(+)、Tr1和CD4(+)CD25(+)FoxP3(+)IL-10(+) T细胞比例高于其他组。我们的研究结果显示，SCALU组未刺激细胞中的IL-10水平升高，并且该组还呈现出以CD4(+) CD25(+)FoxP3(+)细胞群体为主，尽管其中许多细胞为IL-10阴性。
