Sun Guang-Ying, Zhong Dan-Dan, Li Xiang-Jie, Luo Yu-Qing, Ba Hang, Liu Zhao-Sheng, Aisa Haji Akber
Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 40-1 South Beijing Road, Urumqi, 830011, Xinjiang, China.
State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical en Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, Xinjiang, China.
Anal Bioanal Chem. 2015 Sep;407(24):7401-12. doi: 10.1007/s00216-015-8902-0. Epub 2015 Jul 26.
One of the main challenges in the preparation of molecularly imprinted polymers (MIPs) is the substantial initial amount of template needed because of the requirement of high load capacities for most applications. A new strategy of macromolecular crowding was suggested to solve this problem by reducing the amount of template in the polymerization recipe. In a ternary porogenic system of polystyrene (PS) (crowding agent), tetrahydrofuran, and toluene, an imprinted monolithic column with high porosity and good permeability was synthesized using a mixture of ellagic acid (template), acrylamide, and ethylene glycol dimethacrylate. The effect of polymerization factors, including monomer-template molar ratio and the molecular weight and concentration of PS, on the imprinting effect of the resulting MIP monoliths was systematically investigated. At a high ratio of monomer-template (120:1), the greatest imprinting factor of 32.4 was obtained on the MIP monolith with the aid of macromolecular crowding agent. The PS-based imprinted monolith had imprinting even at the extremely high ratio of functional monomer to template of 1510:1. Furthermore, an off-line solid-phase extraction based on the ground MIP was conducted, and the purification recovery of ellagic acid from pomegranate-rind extract was up to 80 %. In conclusion, this approach based on macromolecular crowding is simple, and is especially valuable for those applications of MIP preparation for which a rare template is used.
制备分子印迹聚合物（MIPs）的主要挑战之一是由于大多数应用需要高负载量，因而所需模板的初始量很大。有人提出了一种大分子拥挤的新策略，通过减少聚合配方中模板的量来解决这个问题。在聚苯乙烯（PS）（拥挤剂）、四氢呋喃和甲苯的三元致孔体系中，使用鞣花酸（模板）、丙烯酰胺和乙二醇二甲基丙烯酸酯的混合物合成了具有高孔隙率和良好渗透性的印迹整体柱。系统研究了聚合因素，包括单体与模板的摩尔比以及PS的分子量和浓度，对所得MIP整体柱印迹效果的影响。在单体与模板的比例较高（120:1）时，借助大分子拥挤剂，在MIP整体柱上获得了最大印迹因子32.4。基于PS的印迹整体柱即使在功能单体与模板的比例高达1510:1时仍具有印迹效果。此外，进行了基于研磨后的MIP的离线固相萃取，从石榴皮提取物中鞣花酸的纯化回收率高达80%。总之，这种基于大分子拥挤的方法很简单，对于那些使用稀有模板的MIP制备应用尤其有价值。
