Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 40-1 South Beijing Road, Urumqi 830011, Xinjiang, China; Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
J Chromatogr A. 2012 Aug 17;1251:141-147. doi: 10.1016/j.chroma.2012.06.050. Epub 2012 Jun 20.
Molecular crowding is a new approach to promoting molecular imprinting more efficiently. In this work, this concept was applied to the preparation of imprinted monoliths in the presence of molecular crowding agent for stabilizing binding sites and improving molecular recognition. The imprinted monolithic column was synthesized using a mixture of enrofloxacin (template), methacrylic acid, ethylene glycol dimethacrylate, and polystyrene (molecular crowding agent). Some polymerization factors, such as template-monomer molar ratio and the composition of the porogen, on the imprinting effect of resulting MIP monolith were systematically investigated. Moreover, a stoichiometric displacement model for retention was successfully applied to evaluate the interaction between the solute and the stationary phase. Compared with the MIP prepared by conventional polymerization, the molecular crowding-based monolithic MIPs showed higher selectivity. The results suggested that molecular crowding is a powerful strategy to increase the effect of molecular imprinting.
分子拥挤是一种促进分子印迹更有效的新方法。在这项工作中，该概念被应用于在分子拥挤剂存在下制备印迹整体柱，以稳定结合位点并提高分子识别。印迹整体柱是通过使用恩诺沙星（模板）、甲基丙烯酸、乙二醇二甲基丙烯酸酯和聚苯乙烯（分子拥挤剂）的混合物合成的。系统研究了聚合因素，如模板-单体摩尔比和致孔剂的组成，对所得 MIP 整体柱的印迹效果的影响。此外，成功应用了一种化学计量置换模型来评估溶质与固定相之间的相互作用。与传统聚合制备的 MIP 相比，基于分子拥挤的整体 MIP 表现出更高的选择性。结果表明，分子拥挤是提高分子印迹效果的一种有力策略。
