Peng Hua, He Xiujing, Gao Jian, Ma Haixia, Zhang Zhiming, Shen Yaou, Pan Guangtang, Lin Haijian
Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China; Sichuan Tourism College, Chengdu, 610000, Sichuan, China.
Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute, Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China.
Biochem Biophys Res Commun. 2015 Sep 4;464(4):1040-1047. doi: 10.1016/j.bbrc.2015.07.064. Epub 2015 Jul 23.
The heavy metal cadmium (Cd), acts as a widespread environmental contaminant, which has shown to adversely affect human health, food safety and ecosystem safety in recent years. However, research on how plant respond to various kinds of heavy metal stress is scarcely reported, especially for understanding of complex molecular regulatory mechanisms and elucidating the gene networks of plant respond to Cd stress. Here, transcriptomic changes during Mo17 and B73 seedlings development responsive to Cd pollution were investigated and comparative RNAseq-based approach in both genotypes were performed. 115 differential expression genes (DEGs) with significant alteration in expression were found co-modulated in both genotypes during the maize seedling development; of those, most of DGEs were found comprised of stress and defense responses proteins, transporters, as well as transcription factors, such as thaumatin-like protein, ZmOPR2 and ZmOPR5. More interestingly, genotype-specific transcriptional factors changes induced by Cd stress were found contributed to the regulatory mechanism of Cd sensitivity in both different genotypes. Moreover, 12 co-expression modules associated with specific biological processes or pathways (M1 to M12) were identified by consensus co-expression network. These results will expand our understanding of complex molecular mechanism of response and defense to Cd exposure in maize seedling roots.
重金属镉（Cd）是一种广泛存在的环境污染物，近年来已被证明会对人类健康、食品安全和生态系统安全产生不利影响。然而，关于植物如何应对各种重金属胁迫的研究报道很少，尤其是对于理解复杂的分子调控机制和阐明植物对镉胁迫响应的基因网络。在此，研究了Mo17和B73幼苗发育过程中对镉污染的转录组变化，并对两种基因型进行了基于RNAseq的比较分析。在玉米幼苗发育过程中，发现115个表达有显著变化的差异表达基因（DEGs）在两种基因型中共同调控；其中，大多数差异表达基因由应激和防御反应蛋白、转运蛋白以及转录因子组成，如类甜蛋白、ZmOPR2和ZmOPR5。更有趣的是，发现镉胁迫诱导的基因型特异性转录因子变化有助于两种不同基因型对镉敏感性的调控机制。此外，通过共表达网络鉴定出12个与特定生物过程或途径相关的共表达模块（M1至M12）。这些结果将拓宽我们对玉米幼苗根系对镉暴露响应和防御的复杂分子机制的理解。
