Gao Jian, Zhang Yongzhong, Lu Chaolong, Peng Hua, Luo Mao, Li Gaoke, Shen Yaou, Ding Haiping, Zhang Zhiming, Pan Guangtang, Lin Haijian
Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China.
Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, 611130, China; Sichuan Tourism University, Chengdu, 610000, Sichuan, China.
Biochem Biophys Res Commun. 2015 Mar 6;458(2):287-93. doi: 10.1016/j.bbrc.2015.01.101. Epub 2015 Jan 31.
Lead (Pb), as a heavy metal element, has become the most important metal pollutant of the environment. With allocating a relatively higher proportion of its biomass in roots, maize could be a potential important model to study the phytoremediation of Pb-contaminated soil. Here we analyzed the maize root transcriptome of inbred lines 9782 under heavy metal lead (Pb) pollution, which was identified as a non-hyperaccumulator for Pb in roots. In the present study, more than 98 millions reads were mapped to define gene structure and detect polymorphism, thereby to qualify transcript abundance along roots development under Pb treatment. A total of 17,707, 17,440, 16,998 and 16,586 genes were identified in maize roots at four developmental stages (0, 12 h, 24 h and 48 h) respectively and 2,825, 2,626, 2161 and 2260 stage-specifically expressed genes were also identified respectively. In addition, based on our RNA-Seq data, transcriptomic changes during maize root development responsive to Pb were investigated. A total of 384 differentially expressed genes (DEGs) (log2Ratio ≥ 1, FDR ≤ 0.001) were identified, of which, 36 genes with significant alteration in expression were detected in four developmental stages; 12 DEGs were randomly selected and successful validated by qRT-PCR. Additionally, many transcription factor families might act as the important regulators at different developmental stages, such as bZIP, ERF and GARP et al. These results will expand our understanding of the complex molecular and cellular events in maize root development and provide a foundation for future study on root development in maize under heavy metal pollution and other cereal crops.
铅（Pb）作为一种重金属元素，已成为环境中最重要的金属污染物。玉米在根部分配相对较高比例的生物量，可能是研究铅污染土壤植物修复的潜在重要模式植物。在此，我们分析了重金属铅（Pb）污染下自交系9782的玉米根转录组，该自交系被鉴定为根部铅的非超积累品种。在本研究中，超过9800万条 reads 被定位以确定基因结构并检测多态性，从而确定铅处理下根发育过程中的转录本丰度。在玉米根的四个发育阶段（0、12小时、24小时和48小时）分别鉴定出17707、17440、16998和16586个基因，同时也分别鉴定出2825、2626、2161和2260个阶段特异性表达基因。此外，基于我们的RNA-Seq数据，研究了玉米根发育过程中对铅响应的转录组变化。共鉴定出384个差异表达基因（DEGs）（log2Ratio≥1，FDR≤0.001），其中，在四个发育阶段检测到36个表达有显著变化的基因；随机选择12个DEGs并通过qRT-PCR成功验证。此外，许多转录因子家族可能在不同发育阶段起重要调节作用，如bZIP、ERF和GARP等。这些结果将扩展我们对玉米根发育中复杂分子和细胞事件的理解，并为未来研究重金属污染下玉米及其他谷类作物的根发育提供基础。
