Janus Marleen M, Keijser Bart J F, Bikker Floris J, Exterkate Rob A M, Crielaard Wim, Krom Bastiaan P
a Top Institute Food and Nutrition , Wageningen , the Netherlands.
Biofouling. 2015;31(6):503-10. doi: 10.1080/08927014.2015.1067887.
Commensal oral biofilms, defined by the absence of pathology-related phenotypes, are ubiquitously present. In contrast to pathological biofilms commensal biofilms are rarely studied. Here, the effect of the initial inoculum and subsequent growth conditions on in vitro oral biofilms was studied. Biofilms were inoculated with saliva and grown anaerobically for up to 21 days in McBain medium with or without fetal calf serum (FCS) or sucrose. Pathology-related phenotypes were quantified and the community composition was determined. Biofilms inoculated with pooled saliva or individual inocula were similar. Denaturing gradient gel electrophoresis (DGGE) analysis allowed differentiation of biofilms grown with sucrose, but not with FCS. Lactate production by biofilms was significantly increased by sucrose and protease activity by FCS. McBain grown biofilms showed low activity for both phenotypes. Three clinically relevant in vitro biofilm models were developed and could be differentiated based on pathology-related phenotypes but not DGGE analysis. These models allow analysis of health-to-disease shifts and the effectiveness of prevention measures.
共生口腔生物膜，定义为不存在与病理相关的表型，普遍存在。与病理生物膜相比，共生生物膜很少被研究。在此，研究了初始接种物和后续生长条件对体外口腔生物膜的影响。用唾液接种生物膜，并在含有或不含有胎牛血清（FCS）或蔗糖的 McBain 培养基中厌氧培养长达 21 天。对与病理相关的表型进行定量，并确定群落组成。用混合唾液或个体接种物接种的生物膜相似。变性梯度凝胶电泳（DGGE）分析能够区分用蔗糖培养的生物膜，但不能区分用 FCS 培养的生物膜。蔗糖显著增加了生物膜的乳酸产量，FCS 则增加了蛋白酶活性。在 McBain 培养基中生长的生物膜这两种表型的活性都很低。开发了三种临床相关的体外生物膜模型，可根据与病理相关的表型进行区分，但不能通过 DGGE 分析进行区分。这些模型允许分析从健康到疾病的转变以及预防措施的有效性。
