红细胞悬液长期储存后红细胞膜形态学和纳米结构的紊乱：原子力显微镜研究

Disorders in the Morphology and Nanostructure of Erythrocyte Membranes after Long-term Storage of Erythrocyte Suspension: Atomic Force Microscopy Study.

作者信息

Moroz V V, Chernysh A M, Kozlova E K, Sergunova V A, Gudkova O E, Khoroshilov S E, Onufrievich A D, Kostin A I

机构信息

V. A. Negovsky Research Institute of General Reanimatology, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2015 Jul;159(3):406-10. doi: 10.1007/s10517-015-2975-9. Epub 2015 Jul 28.

DOI:10.1007/s10517-015-2975-9

PMID:26212816

Abstract

Disorders in the erythrocyte morphology and structure of their membranes during long-term storage of erythrocyte suspension (30 days at 4°C) were studied by atomic force microscopy. The morphology and nanostructure of erythrocyte membranes, biochemical parameters, ion exchange parameters, and hemoglobin spectra were recorded. The transformation of erythrocyte morphology and destruction of their membranes were observed throughout the storage period. Irreversible forms of spheroechinocytes and their fragments formed by the end of storage. The concentrations of potassium ions and lactate in solution of the blood preservatiive increased, while pH value decreased. Hemolysis detected by the erythrocyte "leakage" effect was observed starting from days 16-23 of storage.

摘要

通过原子力显微镜研究了红细胞悬液长期储存（4℃下30天）过程中红细胞形态及其膜结构的紊乱情况。记录了红细胞膜的形态和纳米结构、生化参数、离子交换参数以及血红蛋白光谱。在整个储存期间观察到红细胞形态的转变及其膜的破坏。储存结束时形成了不可逆形式的球形棘红细胞及其碎片。血液保存液溶液中的钾离子和乳酸浓度升高，而pH值降低。从储存第16 - 23天开始观察到通过红细胞“渗漏”效应检测到的溶血现象。