Grundy H H, Reece P, Buckley M, Solazzo C M, Dowle A A, Ashford D, Charlton A J, Wadsley M K, Collins M J
Fera, Sand Hutton, York YO41 1LZ, UK.
Fera, Sand Hutton, York YO41 1LZ, UK.
Food Chem. 2016 Jan 1;190:276-284. doi: 10.1016/j.foodchem.2015.05.054. Epub 2015 May 19.
Gelatine is a component of a wide range of foods. It is manufactured as a by-product of the meat industry from bone and hide, mainly from bovine and porcine sources. Accurate food labelling enables consumers to make informed decisions about the food they buy. Since labelling currently relies heavily on due diligence involving a paper trail, there could be benefits in developing a reliable test method for the consumer industries in terms of the species origin of gelatine. We present a method to determine the species origin of gelatines by peptide mass spectrometry methods. An evaluative comparison is also made with ELISA and PCR technologies. Commercial gelatines were found to contain undeclared species. Furthermore, undeclared bovine peptides were observed in commercial injection matrices. This analytical method could therefore support the food industry in terms of determining the species authenticity of gelatine in foods.
明胶是多种食品的组成成分。它是肉类行业从骨头和兽皮中制造出的副产品，主要来源于牛和猪。准确的食品标签能让消费者对所购买的食品做出明智的决定。由于目前的标签严重依赖涉及书面记录的尽职调查，因此开发一种针对明胶物种来源的可靠检测方法对消费行业可能有益。我们提出了一种通过肽质谱法测定明胶物种来源的方法。还与酶联免疫吸附测定法（ELISA）和聚合酶链反应（PCR）技术进行了评估比较。发现市售明胶含有未申报的物种。此外，在市售注射用基质中观察到了未申报的牛肽。因此，这种分析方法在确定食品中明胶的物种真实性方面可为食品行业提供支持。
