Centro de Investigação em Química, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal.
Centro de Investigação em Química, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal.
Food Chem. 2016 Jan 1;190:300-307. doi: 10.1016/j.foodchem.2015.05.065. Epub 2015 May 16.
Proanthocyanidins (PAs) are one of the most important polyphenolic compounds in wine. Among PAs, prodelphinidin (PD) dimers and trimers have not been widely detected in wines due to the lack of available commercial standards and the difficulty to detect and isolate them from natural sources. LC-ESI-MS (liquid chromatography-electrospray ionization-mass spectrometry) with the right chromatographic conditions has proven to be a powerful tool for PAs detection and identification in complex samples. This technique has been applied to an exhaustive study of PA composition of two Portuguese red wines made with teinturier grapes, especially for the identification of PD dimers and trimers. Tandem mass spectrometry (MS/MS) with ion trap provided additional information about the structures of these compounds through the fragmentation patterns of the pseudomolecular ions. A LC-ESI-MS method was optimized and 41 different compounds were found. Among them are included 8 PD dimers and 13 PD trimers.
原花青素（PAs）是葡萄酒中最重要的多酚化合物之一。在 PAs 中，由于缺乏可用的商业标准以及从天然来源中检测和分离它们的困难，普罗德尔宾（PD）二聚体和三聚体在葡萄酒中尚未广泛检测到。带有合适色谱条件的 LC-ESI-MS（液相色谱-电喷雾电离质谱）已被证明是检测和鉴定复杂样品中 PAs 的有力工具。该技术已应用于对用染色葡萄酿造的两种葡萄牙红葡萄酒的 PA 成分进行详尽研究，特别是用于鉴定 PD 二聚体和三聚体。通过准分子离子的碎片模式，带有离子阱的串联质谱（MS/MS）提供了有关这些化合物结构的更多信息。优化了 LC-ESI-MS 方法，发现了 41 种不同的化合物。其中包括 8 种 PD 二聚体和 13 种 PD 三聚体。
