Cui X Y, Chen Z Y, Wu L, Liu X Q, Dong Y Y, Wang F W, Li H Y
College of Life Sciences, Jilin Agricultural University, Changchun, China.
College of Agronomy, Jilin Agricultural University, Changchun, China.
Genet Mol Res. 2015 Jul 3;14(3):7395-405. doi: 10.4238/2015.July.3.15.
The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13- and 7.40-fold higher in leaves than in stems and roots, respectively. Moreover, GUS activity was stimulated by light. In conclusion, spatial and light regulation of the soybean rbcS promoter was observed in N. tabacum, thus illustrating a leaf-specific and light-induced promoter.
利用热不对称交错PCR（TAIL-PCR）和常规PCR克隆了大豆（Glycine max）核酮糖-1,5-二磷酸羧化酶小亚基基因SRS4的调控区，并将其命名为rbcS启动子。该启动子与GUS基因融合，通过农杆菌介导的叶盘转化法导入烟草（Nicotiana tabacum）。在4周龄的转基因烟草植株中，叶片中GUS表达水平最高，叶片中的GUS活性分别比茎和根高7.13倍和7.40倍。此外，光照可刺激GUS活性。总之，在烟草中观察到了大豆rbcS启动子的空间和光调控，从而证明其是一个叶特异性和光诱导型启动子。
