Hu Wen-ting, Qu Hui-hua, Wang Qing-guo, Zhao Yan, Hong Hui, Ren Ya-jun, Liu Wen-ping
Zhong Yao Cai. 2015 Jan;38(1):119-22.
To establish an enzyme linked immunosorbent assay(ELISA) for mangiferin (MRn), artificial antigen of MRn was synthesized.
Oxidation method using sodium iodide was used to synthesize immunogenic antigen (MRn-BSA) and coating antigen(MRn-OVA) of MRn. The characterization of the synthesis was examined by UV spectrometry. BALB/c mice were immunized with the prepared MRn-BSA immunogenic antigen. The titer of the anti-serum was detected by ELISA, in the meanwhile the immunogenicity of MRn-BSA was confirmed by indirect competitive ELISA (icELISA).
UV spectroscopy showed that MRn was successfully conjugated with OVA and BSA,so the new compound of MRn-BSA was synthesized, the coupling ratio was 6: 1. After immuned MRn-BSA, the mice could produce anti-MRn antibodies specifically, of which titer was up to 1: 4 000, and the general range was 1 - 100 µg/mL.
Anti-MRn antibodies are discovered in the serum of mice, which indicates that artificial antigen of MRn is successfully synthesized,for the purpose of preparing monoclonal antibodies, as well as the establishment of appropriate immune methods.
为建立芒果苷（MRn）的酶联免疫吸附测定法（ELISA），合成了MRn人工抗原。
采用碘化钠氧化法合成MRn的免疫原性抗原（MRn-BSA）和包被抗原（MRn-OVA）。通过紫外光谱法对合成产物进行表征。用制备好的MRn-BSA免疫原性抗原免疫BALB/c小鼠。用ELISA检测抗血清效价，同时通过间接竞争ELISA（icELISA）确认MRn-BSA的免疫原性。
紫外光谱显示MRn成功与OVA和BSA偶联，合成了MRn-BSA新化合物，偶联比为6∶1。用MRn-BSA免疫小鼠后，小鼠能特异性产生抗MRn抗体，其效价高达1∶4 000，一般范围为1 - 100 μg/mL。
在小鼠血清中发现了抗MRn抗体，表明成功合成了MRn人工抗原，为制备单克隆抗体以及建立合适的免疫方法奠定了基础。
