Zhang Wei-Ku, Qiao Hao-Yi, Qu Hui-Hua, Zhao Yan, Wang Qing-Guo, Xu Jie-Kun
School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China.
Beijing University of Chinese Medicine "Classical Prescription Application Foundation Research" Innovation Team, Beijing 100029, China.
Zhongguo Zhong Yao Za Zhi. 2016 May;41(10):1880-1883. doi: 10.4268/cjcmm20161020.
Immunogenic antigen (jujuboside A-BSA) and coating antigen (jujuboside A-OVA) of jujuboside A were synthesized by sodium periodate oxidation method for the first time. Jujuboside A artificial antigen was confirmed by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF-MS). The titer and specificity of the antibody in serum of immunized mice were detected by enzyme-linked immunosorbent assay (ELISA). The corrected relation curve of inhibition rate showed that the antibody against Jujuboside A obtained from immunized mice could bind to jujuboside A and the titer was up to 1∶4 000. The jujuboside A artificial antigen was synthesized, which can be used further to preparation of monoclonal antibody and the pharmacokinetics study of jujuboside A in laboratory animals.
首次采用高碘酸钠氧化法合成了酸枣仁皂苷A的免疫原性抗原（酸枣仁皂苷A-BSA）和包被抗原（酸枣仁皂苷A-OVA）。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）对酸枣仁皂苷A人工抗原进行了确证。采用酶联免疫吸附测定（ELISA）检测免疫小鼠血清中抗体的效价和特异性。抑制率校正关系曲线表明，免疫小鼠获得的抗酸枣仁皂苷A抗体能够与酸枣仁皂苷A结合，效价高达1∶4 000。合成了酸枣仁皂苷A人工抗原，可进一步用于制备单克隆抗体以及在实验动物中进行酸枣仁皂苷A的药代动力学研究。
