Shibata Masaki, Sato Tomohito, Taguchi Manzo, Tanaka Atsushi, Harimoto Kouzo, Takeuchi Masaru
Nippon Ganka Gakkai Zasshi. 2015 Jun;119(6):395-401.
To investigate helper T (Th) cell-related cytokines elevated in the vitreous of endogenous uveitis (EU), and to compare these with those of acute retinal necrosis (ARN).
Vitreous fluids were obtained from 23 eyes of EU patients, 4 eyes of ARN patients, and 14 eyes of patients with epiretinal membrane (ERM) who had had vitrectomy surgery. The vitreous levels of interleukin (IL)-1, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, interferon (IFN)-γ, soluble CD40 ligand (sCD40L) and tumor necrosis factor (TNF)-α were measured.
The vitreous cytokine levels of IL-6, IL-10, IL-31, IFN-γ and TNF-α in the EU patients were significantly higher than in the ERM patients. On the other hand, the levels of IL-1β, IL-6, IL-10, IL-31, IFN-γ, sCD40L and TNF-α were significantly higher in the ARN patients than in the EU patients, while those of IL-4, IL-17A and IL-22 were higher in the EU patients than in the ARN patients although they were not significant.
It is suggested that Th1-, Th2- and Th17 cells are related to the development of EU, and Th1- and regulatory T cells to that of ARN, and that the severity of ocular inflammation may be associated with the activity of Th1 cells.
研究内源性葡萄膜炎（EU）患者玻璃体内升高的辅助性T（Th）细胞相关细胞因子，并将其与急性视网膜坏死（ARN）患者的细胞因子进行比较。
收集23例EU患者、4例ARN患者以及14例行玻璃体切割手术的视网膜前膜（ERM）患者的玻璃体液。检测玻璃体内白细胞介素（IL）-1、IL-4、IL-6、IL-10、IL-17A、IL-17F、IL-21、IL-22、IL-23、IL-25、IL-31、IL-33、干扰素（IFN）-γ、可溶性CD40配体（sCD40L）和肿瘤坏死因子（TNF）-α的水平。
EU患者玻璃体内IL-6、IL-10、IL-31、IFN-γ和TNF-α的细胞因子水平显著高于ERM患者。另一方面，ARN患者玻璃体内IL-1β、IL-6、IL-10、IL-31、IFN-γ、sCD40L和TNF-α的水平显著高于EU患者，而EU患者玻璃体内IL-4、IL-17A和IL-22的水平虽高于ARN患者但差异无统计学意义。
提示Th1、Th2和Th17细胞与EU的发生发展有关，Th1和调节性T细胞与ARN的发生发展有关，且眼部炎症的严重程度可能与Th1细胞的活性有关。
