葡萄膜炎患者眼液样本中免疫调节细胞因子的分析。

Analysis of immunoregulatory cytokines in ocular fluid samples from patients with uveitis.

作者信息

Ongkosuwito J V, Feron E J, van Doornik C E, Van der Lelij A, Hoyng C B, La Heij E C, Kijlstra A

机构信息

Department of Ophthalmology, Academic Medical Centre, University of Amsterdam, The Netherlands.

出版信息

Invest Ophthalmol Vis Sci. 1998 Dec;39(13):2659-65.

PMID:9856775

Abstract

PURPOSE

To investigate the T-helper cell cytokine profiles in two well-defined clinical uveitis entities caused by an infectious mechanism.

METHODS

Cytokines (interleukin [IL]-2, IL-4, IL-6, IL-10, and interferon [IFN]-gamma) were measured in ocular fluid samples obtained from patients with herpes simplex- or varicella-zoster virus-induced acute retinal necrosis (ARN; n = 17) and toxoplasma chorioretinitis (n = 27) using enzyme-linked immunosorbent assay techniques. The data were compared with data for 51 control samples taken during cataract surgery (n = 10), vitrectomy in diabetic retinopathy (n = 10), eye bank eyes (n = 10) and with samples from patients with "autoimmune" uveitis (n = 21).

RESULTS

Interleukin-6 was detected in 44 of 51 control samples and 43 of 44 eyes of patients with uveitis. The highest levels in the control samples were detected in 9 of 10 vitreous samples from patients with diabetic retinopathy (mean, 648 pg/ml). In 8 of 10 samples taken from patients during cataract surgery and in 7 of 10 eye bank eyes the amount of IL-6 was significantly lower (mean, 10 pg/ml and 136 pg/ml, respectively). Interleukin-6 levels in patients with ARN (mean, 1436 pg/ml) were significantly higher than in those with toxoplasma chorioretinitis (mean, 272 pg/ml). Interleukin-2 was detected in one of the samples from patients with toxoplasma chorioretinitis (1105 pg/ml) and in three samples from the control subjects suffering from Fuchs' heterochromic anterior uveitis (mean, 752 pg/ml). No IL-4 (<2 pg/ml) was detected either in patient or control samples. Interferon-gamma could be detected in 7 of 17 ARN patients (range, 277-3483 pg/ml), in 13 of 27 samples from patients with toxoplasma chorioretinitis (range, 12-250 pg/ml), and in 1 of 21 of the samples from control subjects with uveitis (31 pg/ml) but was absent in nonuveitic control samples. Interleukin-10 was detected in 10 of 17 ARN patients (range, 29-3927 pg/ml), in 13 of 27 samples from patients with toxoplasma chorioretinitis (range, 4-67 pg/ml), and in only 3 of 51 control samples (6 pg/ml, 16 pg/ml, and 20 pg/ml).

CONCLUSIONS

Various immunoregulatory cytokines (IL-6, IL-10, and IFN-gamma) were detected in ocular fluid samples from patients with uveitis. A separate role for either a T-helper type 1 or T-helper type 2 response in the pathogenesis of clinical uveitis could not be proven.

摘要

目的

研究由感染机制引起的两种明确的临床葡萄膜炎实体中辅助性T细胞细胞因子谱。

方法

使用酶联免疫吸附测定技术，对从单纯疱疹病毒或水痘带状疱疹病毒引起的急性视网膜坏死（ARN；n = 17）和弓形虫脉络膜视网膜炎（n = 27）患者获得的眼液样本中的细胞因子（白细胞介素[IL]-2、IL-4、IL-6、IL-10和干扰素[IFN]-γ）进行测量。将数据与白内障手术期间采集的51份对照样本（n = 10）、糖尿病性视网膜病变玻璃体切除术样本（n = 10）、眼库眼样本（n = 10）以及“自身免疫性”葡萄膜炎患者样本（n = 21）的数据进行比较。

结果

在51份对照样本中的44份以及葡萄膜炎患者的44只眼中检测到白细胞介素-6。对照样本中最高水平在糖尿病性视网膜病变患者的10份玻璃体样本中的9份中检测到（平均，648 pg/ml）。在白内障手术患者的10份样本中的8份以及眼库眼的10份样本中的7份中，IL-6的量显著较低（平均分别为10 pg/ml和136 pg/ml）。ARN患者的白细胞介素-6水平（平均，1436 pg/ml）显著高于弓形虫脉络膜视网膜炎患者（平均，272 pg/ml）。在弓形虫脉络膜视网膜炎患者的一份样本（1105 pg/ml）以及患有富克斯异色性前葡萄膜炎的对照受试者的三份样本（平均，752 pg/ml）中检测到白细胞介素-2。在患者或对照样本中均未检测到IL-4（<2 pg/ml）。在17例ARN患者中的7例（范围，277 - 3483 pg/ml）、27例弓形虫脉络膜视网膜炎患者样本中的13例（范围，12 - 250 pg/ml）以及21例葡萄膜炎对照受试者样本中的1例（31 pg/ml）中检测到干扰素-γ，但在非葡萄膜炎对照样本中未检测到。在17例ARN患者中的10例（范围，29 - 3927 pg/ml）、27例弓形虫脉络膜视网膜炎患者样本中的13例（范围，4 - 67 pg/ml）以及51份对照样本中的仅3份（6 pg/ml、16 pg/ml和20 pg/ml）中检测到白细胞介素-10。