Nogina T M, Dumanskaya T U, Homenko L A, Kisten A G
Mikrobiol Z. 2015 May-Jun;77(3):47-52.
It has been established that the presence in the medium Ca2+ and Fe2+ ions in 1.2 - 1.3 times increases the assimilation of hydrocarbon by actinobacteria. The possibility of joint growth of binary actinobacteria cultures on mineral medium with diesel fuel without reducing the assimilation of this substrate compared to monocultures it was shown. In the conditions of co-batch culture Rhodococcus erythropolis IMV B-7277 and Dietzia maris IMV B-7278 strains in the laboratory fermenter "Biotec" (stirring speed 500 r.p.m., degree of aeration 0.3 1/1 per min, sulfite number 1.5 g O2 /1 h,) on the medium with 1.0% diesel oil in the presence of 4 mg/l Ca2+, Fe2+ and 1 g/l yeast extract for 30 hours the substrate assimilation rate was 87.8% and the concentration of biomass - 2.0 g/l.
已经确定，培养基中Ca2+和Fe2+离子的存在会使放线菌对碳氢化合物的同化作用提高1.2 - 1.3倍。研究表明，与单一培养相比，二元放线菌培养物在含有柴油的矿物培养基上共同生长时，不会降低对该底物的同化作用。在实验室发酵罐“Biotec”（搅拌速度500转/分钟，通气量0.3升/升每分钟，亚硫酸盐值1.5克O2 /升小时）中，在含有1.0%柴油的培养基上，在4毫克/升Ca2+、Fe2+和1克/升酵母提取物存在的条件下，将红平红球菌IMV B - 7277和海迪茨氏菌IMV B - 7278菌株进行共批培养30小时，底物同化率为87.8%，生物量浓度为2.0克/升。
