Yudina N Yu, Arlyapov V A, Chepurnova M A, Alferov S V, Reshetilov A N
Federal State Budgetary Educational Institution of Higher Education "Tula State University", 92 Lenin Prosp., Tula 300012, Russia.
Federal State Budgetary Institution of Science "G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms", Russian Academy of Sciences, 5 Prosp. Nauki, Pushchino, Moscow Region 142290, Russia; Federal State Budgetary Educational Institution of Higher Education "Tula State University", 92 Lenin Prosp., Tula 300012, Russia.
Enzyme Microb Technol. 2015 Oct;78:46-53. doi: 10.1016/j.enzmictec.2015.06.008. Epub 2015 Jun 11.
Artificial microbial co-cultures were formed to develop the receptor element of a biosensor for assessment of biological oxygen demand (BOD). The co-cultures possessed broad substrate specificities and enabled assays of water and fermentation products within a broad BOD range (2.4-80 mg/dm(3)) with a high correlation to the standard method (R = 0.9988). The use of the co-cultures of the yeasts Pichia angusta, Arxula adeninivorans and Debaryomyces hansenii immobilized in N-vinylpyrrolidone-modified poly(vinyl alcohol) enabled developing a BOD biosensor possessing the characteristics not inferior to those in the known biosensors. The results are indicative of a potential of using these co-cultures as the receptor element base in prototype models of instruments for broad application.
构建人工微生物共培养体系，以开发用于评估生物需氧量（BOD）的生物传感器的受体元件。该共培养体系具有广泛的底物特异性，能够在较宽的BOD范围内（2.4 - 80 mg/dm³）对水和发酵产物进行测定，与标准方法具有高度相关性（R = 0.9988）。将酵母奥古斯塔毕赤酵母、嗜腺嘌呤阿苏鲁酵母和汉逊德巴利酵母固定在N - 乙烯基吡咯烷酮改性的聚乙烯醇中形成的共培养体系，能够开发出性能不低于已知生物传感器的BOD生物传感器。结果表明，这些共培养体系有潜力作为广泛应用的仪器原型模型中的受体元件基础。
