Zhao Lei, He Li, Chen Shujuan, Zou Likou, Zhou Kang, Ao Xiaolin, Liu Shuliang, Hu Xinjie, Han Guoquan
College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
Enzyme Microb Technol. 2017 Mar;98:52-57. doi: 10.1016/j.enzmictec.2016.11.010. Epub 2016 Nov 23.
Biochemical oxygen demand (BOD) sensors based on Zr (IV)-loaded collagen fiber (ZrCF), a novel material with great porous structure, were developed. This novel material shows adsorbability by microorganisms. Saccharomyces cerevisiae and Escherichia coli were used for the construction of BOD sensors. Factors affecting BOD sensor performance were examined. The ZrCF-based BOD sensor showed different sensitivities and linear response ranges with different biofilm densities. The amount of microorganisms strongly affected the performance of the BOD sensor. Poor permeability of previously reported immobilization carriers were greatly circumvented by ZrCF. The service life of the ZrCF-based BOD sensor was more than 42 days. The immobilized microorganisms can be stored for more than 6 months under 4°C in PB solution. There was good correlation between the results of the sensor method and the standard 5-day BOD method in the determination of pure organic substrates and real water samples.
基于负载锆（IV）的胶原纤维（ZrCF）开发了生化需氧量（BOD）传感器，ZrCF是一种具有大孔结构的新型材料。这种新型材料显示出对微生物的吸附性。酿酒酵母和大肠杆菌被用于构建BOD传感器。研究了影响BOD传感器性能的因素。基于ZrCF的BOD传感器在不同生物膜密度下表现出不同的灵敏度和线性响应范围。微生物数量强烈影响BOD传感器的性能。ZrCF极大地克服了先前报道的固定化载体渗透性差的问题。基于ZrCF的BOD传感器的使用寿命超过42天。固定化微生物在4°C下于PB溶液中可保存6个月以上。在测定纯有机底物和实际水样时，传感器方法的结果与标准五日BOD方法的结果之间具有良好的相关性。
