Pertsov S S, Kalinichenko L S, Koplik E V, Nagler L G, Alinkina E S, Kozachenko A I
Anokhin Research Institute of Normal Physiology, Moscow, Russia.
Emanuel' Institute of Biochemical Physics, Moscow, Russia.
Biomed Khim. 2015 May-Jun;61(3):394-9. doi: 10.18097/PBMC20156103394.
The effect of the epiphyseal hormone melatonin on the activity of antioxidant enzymes, glutathione peroxidase (GPx), glutathione reductase (GR), and Cu/Zn-superoxide dismutase (Cu/Zn-SOD) was studied in peripheral blood erythrocytes of behaviorally passive and active Wistar rats. Acute emotional stress was modeled by immobilization of animals for1 h with simultaneous electrocutaneous stimulation. Basal activity of antioxidant glutathione enzymes in erythrocytes of behaviorally passive rats was higher than that in active animals. Administration of melatonin (2 mg/kg, intraperitoneally) was accompanied by a decrease in the activity of GPx and GR in erythrocytes from non-stressed passive animals. After experimental stress, passive rats demonstrated a significant increase in the activity of Cu/Zn-SOD and GPx in peripheral blood erythrocytes. The absence of stress-induced changes in functional activity of antioxidant defense enzymes in the blood of behaviorally active animals suggests a relatively constant oxidative status of tissues in these animals under stress conditions. Melatonin administration had little effect on stress-induced changes in functional activity of the erythrocyte antioxidant system in passive rats. Active specimens pretreated with melatonin before stress exposure were characterized by activation of study antioxidant enzymes. Quantitative parameters of the erythrocyte antioxidant defense enzymes did not differ in behaviorally active and passive rats subjected to experimental stress after melatonin injection. Thus, exogenous melatonin abolishes differences in the activity of study antioxidant enzymes in erythrocytes of animals with different behavioral parameters under basal conditions and after experimental stress. In passive rats melatonin mainly reduced the initial tension of oxidative processes. By contrast, administration of this hormone to active specimens is followed by an increase in functional activity of the antioxidant enzyme system under conditions of acute stress.
在行为被动和活跃的Wistar大鼠外周血红细胞中，研究了骨骺激素褪黑素对抗氧化酶、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）和铜/锌超氧化物歧化酶（Cu/Zn-SOD）活性的影响。通过将动物固定1小时并同时进行皮肤电刺激来模拟急性情绪应激。行为被动大鼠红细胞中抗氧化谷胱甘肽酶的基础活性高于活跃动物。给非应激被动动物腹腔注射褪黑素（2mg/kg）后，红细胞中GPx和GR的活性降低。实验应激后，被动大鼠外周血红细胞中Cu/Zn-SOD和GPx的活性显著增加。行为活跃动物血液中抗氧化防御酶的功能活性在应激后没有变化，这表明这些动物在应激条件下组织的氧化状态相对恒定。褪黑素给药对被动大鼠红细胞抗氧化系统功能活性的应激诱导变化影响较小。在应激暴露前用褪黑素预处理的活跃样本表现为研究中的抗氧化酶被激活。在注射褪黑素后经受实验应激的行为活跃和被动大鼠中，红细胞抗氧化防御酶的定量参数没有差异。因此，外源性褪黑素消除了基础条件下和实验应激后具有不同行为参数的动物红细胞中研究的抗氧化酶活性的差异。在被动大鼠中，褪黑素主要降低氧化过程的初始张力。相比之下，在急性应激条件下，给活跃样本注射这种激素后，抗氧化酶系统的功能活性会增加。
