Kalinichenko L S, Pertsov S S, Koplik E V
P. K. Anokhin Research Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia.
Bull Exp Biol Med. 2013 Nov;156(1):7-10. doi: 10.1007/s10517-013-2264-4.
We studied the effect of IL-4 on antioxidant enzyme activity in brain structures (hypothalamus, sensorimotor cortex, and amygdala) in behaviorally passive and active rats. One-hour immobilization of animals with simultaneous delivery of subthreshold electrocutaneous stimulation was used as the model of acute stress. Intraperitoneal injection of IL-4 (5 μg/kg) was followed by an increase in activities of glutathione peroxidase and Cu/Zn SOD in the hypothalamus of non-stressed rats. Activities of glutathione peroxidase and Cu/Zn SOD in the amygdala were shown to decrease. Administration of IL-4 was accompanied by activation of glutathione peroxidase (active and passive rats), glutathione reductase (passive rats), and Cu/Zn SOD (active rats) in the sensorimotor cortex. These data indicate that the efficiency of antioxidant protection increases in the hypothalamus and sensorimotor cortex, but decreases in the amygdala of rats receiving IL-4. Pretreatment with IL-4 abolished a poststress increase in glutathione peroxidase activity in the sensorimotor cortex of passive animals.
我们研究了白细胞介素-4（IL-4）对行为被动和主动的大鼠脑结构（下丘脑、感觉运动皮层和杏仁核）中抗氧化酶活性的影响。将动物固定1小时并同时给予阈下电皮肤刺激作为急性应激模型。对非应激大鼠腹腔注射IL-4（5μg/kg）后，下丘脑谷胱甘肽过氧化物酶和铜/锌超氧化物歧化酶（Cu/Zn SOD）的活性增加。杏仁核中谷胱甘肽过氧化物酶和Cu/Zn SOD的活性降低。在感觉运动皮层中，给予IL-4伴随着谷胱甘肽过氧化物酶（主动和被动大鼠）、谷胱甘肽还原酶（被动大鼠）和Cu/Zn SOD（主动大鼠）的激活。这些数据表明，接受IL-4的大鼠下丘脑和感觉运动皮层中抗氧化保护效率增加，但杏仁核中抗氧化保护效率降低。IL-4预处理消除了被动动物感觉运动皮层应激后谷胱甘肽过氧化物酶活性的增加。
