来自中国的两种葡萄扇叶病毒相关病毒分离株的分子特征分析
Molecular characterizations of two grapevine rupestris stem pitting-associated virus isolates from China.
作者信息
Hu Guo-Jun, Dong Ya-Feng, Zhu Hong-Juan, Zhang Zun-Ping, Fan Xu-Dong, Ren Fang, Zhou Jun
机构信息
National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, People's Republic of China.
出版信息
Arch Virol. 2015 Oct;160(10):2641-5. doi: 10.1007/s00705-015-2544-6. Epub 2015 Jul 29.
The complete nucleotide sequences of two isolates of grapevine rupestris stem pitting-associated virus (LSL and JF) collected from grapevine of Xingcheng in Liaoning Province, China, were determined. The genomes of both LSL and JF were found to contain five open reading frames (ORFs). Sequence alignments showed that the genomic sequences of JF were 76.1 %-83.5 % identical to the other ten GRSPaV isolates that have been reported previously and that the nucleotide sequence identity of isolate LSL to other isolates was no more than 78 %. Phylogenetic analysis based on the complete genome sequence indicated that JF belongs to group III and that LSL belongs to a new group (group IV). The average genetic distances of the new genetic lineage from groups I, II and III were 0.34, 0.32 and 0.33, respectively.
测定了从中国辽宁省兴城葡萄上采集的两株葡萄扇叶粗皮病相关病毒（LSL和JF）的完整核苷酸序列。发现LSL和JF的基因组均包含五个开放阅读框（ORF）。序列比对表明，JF的基因组序列与先前报道的其他十株葡萄扇叶粗皮病相关病毒分离株的序列一致性为76.1%-83.5%，而分离株LSL与其他分离株的核苷酸序列一致性不超过78%。基于完整基因组序列的系统发育分析表明，JF属于第三组，LSL属于一个新组（第四组）。新遗传谱系与第一、二、三组的平均遗传距离分别为0.34、0.32和0.33。
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