Dipartimento di Biologia e Chimica Agroforestale ed Ambientale, Università degli Studi di Bari ed Istituto di Virologia Vegetale del CNR UOS-Bari, via Amendola 165/A, 70126 Bari, Italy.
Virus Res. 2012 Jan;163(1):262-8. doi: 10.1016/j.virusres.2011.10.010. Epub 2011 Oct 20.
Field symptoms of chlorotic mottling and leaf deformations were observed on the cv Pinot gris (PG) in the Trentino region (Italy). Extensive assays excluded the presence of widely distributed nepo-, ampelo- and vitiviruses. An analysis of small RNA populations from two PG grapevines showing or not symptoms was carried out by Illumina high throughput sequencing. The study disclosed the virus and viroids contents of the two vines that was composed by Grapevine rupestris stem pitting-associated virus (GRSPaV), two viroids Hop stunt viroid (HSVd) and Grapevine yellow speckle viroid 1 (GYSVd1), the marafiviruses Grapevine rupestris vein feathering virus (GRVFV) and Grapevine Syrah virus 1 (GSyV-1), and a hitherto unrecorded virus. This virus had a genome organization identical to that of Grapevine berry inner necrosis virus (GINV), a trichovirus reported only from Japan, with which it grouped in phylogenetic trees constructed with sequences of the RdRp domain and the coat protein gene. However, molecular differences with GINV are wide enough to warrant classification of the virus in question as a new species, for which the provisional name of Grapevine Pinot gris virus (GPGV) is proposed. A limited field survey for the presence of GPGV in diseased and symptomless plants from three different cultivars did not allow to clearly associating the virus to the observed symptoms.
在意大利特伦蒂诺地区（意大利）的 Pinot gris（PG）品种上观察到叶片褪绿斑驳和畸形等田间症状。广泛的检测排除了广泛分布的 Nepo、Ampelo 和 Vitiviruses 的存在。对表现或不表现症状的两个 PG 葡萄藤的小 RNA 群体进行了 Illumina 高通量测序分析。研究揭示了两个葡萄藤的病毒和类病毒含量，由葡萄岩蚰坑伴随病毒（GRSPaV）、两种类病毒 Hop 发育迟缓病毒（HSVd）和葡萄黄斑类病毒 1（GYSVd1）、Marafiviruses 葡萄岩蚰脉纹病毒（GRVFV）和 Syrah 葡萄病毒 1（GSyV-1）以及一种迄今未记录的病毒组成。该病毒的基因组组织与仅在日本报道的 Grapevine berry inner necrosis virus（GINV）相同，与该病毒在 RdRp 结构域和外壳蛋白基因序列构建的系统进化树中分组。然而，与 GINV 的分子差异足够大，足以将该病毒分类为一个新种，暂定名为 Grapevine Pinot gris virus（GPGV）。对来自三个不同品种的患病和无症状植物中 GPGV 存在情况的有限田间调查未能明确将该病毒与观察到的症状联系起来。
