Ilgūnas Mikas, Palinauskas Vaidas, Iezhova Tatjana A, Valkiūnas Gediminas
Institute of Ecology, Nature Research Centre, Akademijos 2, Vilnius 2100, LT-08412, Lithuania.
Zootaxa. 2013;3666:49-61. doi: 10.11646/zootaxa.3666.1.5.
Plasmodium hoionucleophilum n. sp. was described from the Common Grasshopper Warbler Locustella naevia based on the morphology of blood stages and partial sequences of the mitochondrial cytochrome b (cyt b) gene. This malaria parasite belongs to the subgenus Novyella; it can be readily distinguished from all described Novyella parasites due to two features, i. e. the strict adherence of its meronts to the nuclei of infected erythrocytes and the lack of such adherence in the case of gametocytes. We also found the lineage pLZFUS01 in Red-Backed Shrike Lanius collurio, identified this parasite and conclude that it belongs to Plasiodium relictum. Illustrations of blood stages of these two parasites are given. DNA lineages associated with P. hoionucleophilum (pSW2, GenBank KC342643) and P. relictum (pLZFUS01, GenBank KC342644) are reported and can be used for molecular identification of these malarial infections. Phylogenetic analysis determines DNA lineages closely related to both reported parasites and is in accordance with the parasites' morphological identification. This study contributes to barcoding of avian malaria parasites using partial sequences of cyt b gene.
嗜核疟原虫新种（Plasmodium hoionucleophilum n. sp.）是根据血期形态和线粒体细胞色素b（cyt b）基因的部分序列，从普通草莺（Locustella naevia）中描述出来的。这种疟原虫属于诺维耶拉亚属（Novyella）；由于两个特征，它很容易与所有已描述的诺维耶拉疟原虫区分开来，即其裂殖体严格附着于受感染红细胞的细胞核，而配子体则缺乏这种附着。我们还在红背伯劳（Lanius collurio）中发现了谱系pLZFUS01，鉴定了这种寄生虫，并得出结论它属于残疟原虫（Plasiodium relictum）。给出了这两种寄生虫血期的图示。报告了与嗜核疟原虫（pSW2，GenBank KC342643）和残疟原虫（pLZFUS01，GenBank KC342644）相关的DNA谱系，可用于这些疟疾感染的分子鉴定。系统发育分析确定了与这两种已报道寄生虫密切相关的DNA谱系，并且与寄生虫的形态学鉴定结果一致。本研究有助于利用cyt b基因的部分序列对鸟类疟原虫进行条形码鉴定。
