盐度对河口沉积物中 AOA 和 AOB 的丰度、转录活性和多样性的影响：一项微宇宙研究。
The influence of salinity on the abundance, transcriptional activity, and diversity of AOA and AOB in an estuarine sediment: a microcosm study.
机构信息
School of Environment, Tsinghua University, Beijing, 100084, China.
Department of Civil and Environmental Engineering, University of Alberta, Edmonton, T6G 2 W2, Canada.
出版信息
Appl Microbiol Biotechnol. 2015 Nov;99(22):9825-33. doi: 10.1007/s00253-015-6804-x. Epub 2015 Jul 29.
Estuarine sediment-seawater microcosms were established to evaluate the influence of salinity on the population, transcriptional activity, and diversity of ammonia-oxidizing archaea (AOA) and bacteria (AOB). AOA was found to show the most abundant and the highest transcriptional activity under moderate salinity; on the other hand, AOB abundance was not sensitive to salinity variation but showed the highest transcriptional activity in the low-salinity microcosms. AOA exhibited more advantages than AOB on growth and ammonia-oxidizing activity under moderate- and high-salinity environments. The highest richness and diversity of active AOA were found under salinity of 15 psu. All the active AOA detected under the salinities studied were clustered into Nitrosopumilus maritimus linage, with the composition shifted from N. maritimus C12 cluster, N. maritimus like 1.1 cluster, N. maritimus SCM1 cluster, and N. maritimus like 1.2 cluster to N. maritimus C12 and N. maritimus A10 clusters when salinity was increased from 5 to 30 psu.
建立了河口沉积物-海水微宇宙，以评估盐度对氨氧化古菌（AOA）和细菌（AOB）的种群、转录活性和多样性的影响。结果表明，在中等盐度下，AOA 的丰度最高，转录活性最强；另一方面，AOB 的丰度对盐度变化不敏感，但在低盐度微宇宙中表现出最高的转录活性。在中高盐度环境下，AOA 在生长和氨氧化活性方面比 AOB 具有更多优势。在盐度为 15 psu 时，发现活性 AOA 的丰富度和多样性最高。在所研究的盐度下检测到的所有活性 AOA 均聚类为 Nitrosopumilus maritimus 谱系，组成从 N. maritimus C12 聚类、N. maritimus like 1.1 聚类、N. maritimus SCM1 聚类和 N. maritimus like 1.2 聚类转变为 N. maritimus C12 和 N. maritimus A10 聚类，当盐度从 5 增加到 30 psu 时。
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