State Key Laboratory of Biocontrol/Southern Marine Sciences and Engineering Guangdong Laboratory (Zhuhai), School of Marine Sciences/School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
J Appl Microbiol. 2021 May;130(5):1442-1455. doi: 10.1111/jam.14846. Epub 2020 Oct 20.
Ammonia oxidation is a significant process of nitrogen cycles in a lot of ecosystems sediments while there are few studies in shrimp culture pond (SCP) sediments. This paper attempted to explore the community diversity and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in SCP sediments at different culture stages.
We collected SCP sediments and analysed the community diversity and abundance of AOA and bacteria in shrimp pond sediment at different culture stages using the ammonia monooxygenase (amoA) gene with quantitative PCR (qPCR) and 16S rRNA gene sequencing. The AOB-amoA gene abundance was showed higher than AOA-amoA gene abundance in SCP sediments on Day 50 and Day 60 after shrimp larvae introducing into the pond, and the diversity of AOA in SCP sediments was higher than that of AOB. The phylogenetic tree revealed that the most of AOA were the member of Nitrosopumilus and Nitrososphaera, and the majority of AOB sequences were clustered into Nitrosospira, Nitrosomonas clusters 6a and 7. The AOA community has close relationship with total organic carbon (TOC), pH, total phosphorus (TP), nitrate reductase, urease, acid phosphatase and β-glucosidase. The AOB community was related to TOC, C/N and nitrate reductase.
AOA and AOB play the different ecological roles in SCP sediments at different culture stages.
Our results suggested that the different community diversity and abundance of AOA and AOB in SCP sediments, which may improve our ecological cognition of shrimp culture stages in SCP ecosystems.
在许多生态系统沉积物中，氨氧化是氮循环的重要过程，但在虾养殖池塘（SCP）沉积物中研究较少。本文试图探讨不同养殖阶段 SCP 沉积物中氨氧化古菌（AOA）和氨氧化细菌（AOB）的群落多样性和丰度。
我们收集了 SCP 沉积物，并使用定量 PCR（qPCR）和 16S rRNA 基因测序分析了虾苗引入池塘后不同养殖阶段 SCP 沉积物中 AOA 和细菌的群落多样性和丰度。在虾苗引入池塘后的第 50 天和第 60 天，SCP 沉积物中的 AOB-amoA 基因丰度高于 AOA-amoA 基因丰度，且 SCP 沉积物中 AOA 的多样性高于 AOB。系统发育树显示，大多数 AOA 是 Nitrosopumilus 和 Nitrososphaera 的成员，而大多数 AOB 序列聚类为 Nitrosospira、Nitrosomonas 6a 和 7 簇。AOA 群落与总有机碳（TOC）、pH 值、总磷（TP）、硝酸盐还原酶、脲酶、酸性磷酸酶和β-葡萄糖苷酶密切相关。AOB 群落与 TOC、C/N 和硝酸盐还原酶有关。
在不同的养殖阶段，SCP 沉积物中的 AOA 和 AOB 发挥着不同的生态作用。
我们的研究结果表明，SCP 沉积物中 AOA 和 AOB 的群落多样性和丰度不同，这可能提高了我们对 SCP 生态系统中虾养殖阶段的生态认知。
