Haghshenas Babak, Nami Yousef, Haghshenas Minoo, Abdullah Norhafizah, Rosli Rozita, Radiah Dayang, Khosroushahi Ahmad Yari
Institute of Bioscience, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Microbiologyopen. 2015 Oct;4(5):803-13. doi: 10.1002/mbo3.280. Epub 2015 Jul 27.
This study aimed to find candidate strains of Lactobacillus isolated from sheep dairy products (yogurt and ewe colostrum) with probiotic and anticancer activity. A total of 100 samples were randomly collected from yogurt and colostrum and 125 lactic acid bacteria were isolated. Of these, 17 Lactobacillus strains belonging to five species (L. delbrueckii, L. plantarum, L. rhamnosus, L. paracasei, and L. casei) were identified. L. plantarum 17C and 13C, which isolated from colostrums, demonstrated remarkable results such as resistant to low pH and high concentrations of bile salts, susceptible to some antibiotics and good antimicrobial activity that candidate them as potential probiotics. Seven strains (1C, 5C, 12C, 13C, 17C, 7M, and 40M), the most resistant to simulated digestion, were further investigated to evaluate their capability to adhere to human intestinal Caco-2 cells. L. plantarum 17C was the most adherent strain. The bioactivity assessment of L. plantarum 17C showed anticancer effects via the induction of apoptosis on HT-29 human cancer cells and negligible side effects on one human epithelial normal cell line (FHs 74). The metabolites produced by this strain can be used as alternative pharmaceutical compounds with promising therapeutic indices because they are not cytotoxic to normal mammalian cells.
本研究旨在从羊乳制品（酸奶和初乳）中寻找具有益生菌和抗癌活性的乳酸杆菌候选菌株。共从酸奶和初乳中随机采集了100个样本，分离出125株乳酸菌。其中，鉴定出属于五个物种（德氏乳杆菌、植物乳杆菌、鼠李糖乳杆菌、副干酪乳杆菌和干酪乳杆菌）的17株乳酸杆菌菌株。从初乳中分离出的植物乳杆菌17C和13C表现出显著的结果，如耐低pH值和高浓度胆盐、对某些抗生素敏感以及良好的抗菌活性，使其成为潜在益生菌的候选菌株。对七株对模拟消化最具抗性的菌株（1C、5C、12C、13C、17C、7M和40M）进一步研究，以评估它们黏附于人肠道Caco-2细胞的能力。植物乳杆菌17C是黏附性最强的菌株。植物乳杆菌17C的生物活性评估显示，它通过诱导HT-29人癌细胞凋亡发挥抗癌作用，而对一种人上皮正常细胞系（FHs 74）的副作用可忽略不计。该菌株产生的代谢产物可作为具有良好治疗指标的替代药物化合物，因为它们对正常哺乳动物细胞无细胞毒性。
