Koncan R, Parisato M, Sakarikou C, Stringari G, Fontana C, Favuzzi V, Ligozzi M, Lo Cascio G
Department of Pathology and Diagnostics, University of Verona, Strada Le Grazie 8, 37134, Verona, Italy.
O.U. of Microbiology and Virology, Department of Pathology and Diagnostics, Azienda Ospedaliera Universitaria Integrata di Verona, P.le A. Scuro 10, 37134, Verona, Italy.
Eur J Clin Microbiol Infect Dis. 2015 Oct;34(10):2097-102. doi: 10.1007/s10096-015-2458-y. Epub 2015 Jul 29.
Rapid detection of microorganisms in respiratory specimens is of paramount importance to drive the proper antibiotic regimen to prevent complications and transmission of infections. In the present study, the respiFISH® HAP Gram (-) Panel (miacom diagnostics GmbH, Duesseldorf, Germany) for the etiological diagnosis of hospital-acquired pneumonia was compared with the traditional culture method for the detection of major Gram-negative pathogens in respiratory specimens. respiFISH® combined the classical fluorescence in situ hybridization (FISH) technology with fluorescence-labeled DNA molecular beacons as probes. From September 2011 to January 2012, 165 samples were analyzed: the sensitivity and specificity were 94.39 and 87.93%, respectively. Only six pathogens (3.6%) were not identified with respiFISH®, while seven specimens (3%) provided false-positive results. This beacon-based identification shortens the time to result by at least one work day, providing species-level identification within half an hour. Considering the high sensitivity and specificity and the significant time saving, the introduction of bbFISH® assays could effectively complement traditional systems in microbiology laboratories.
快速检测呼吸道标本中的微生物对于确定合适的抗生素治疗方案以预防感染并发症和传播至关重要。在本研究中，将用于医院获得性肺炎病因诊断的respiFISH® HAP革兰氏阴性菌检测试剂盒（德国杜塞尔多夫miacom诊断有限公司）与传统培养方法进行比较，以检测呼吸道标本中的主要革兰氏阴性病原体。respiFISH® 将经典的荧光原位杂交（FISH）技术与荧光标记的DNA分子信标作为探针相结合。在2011年9月至2012年1月期间，共分析了165份样本：敏感性和特异性分别为94.39%和87.93%。只有六种病原体（3.6%）未被respiFISH® 检测到，而七个样本（3%）出现假阳性结果。这种基于信标的鉴定方法将出结果时间至少缩短了一个工作日，半小时内即可实现菌种水平的鉴定。鉴于其高敏感性和特异性以及显著的时间节省，引入bbFISH® 检测方法可有效补充微生物实验室的传统检测系统。
