Baurmann H, Klamann A, Wolfrum G
Universitäts-Augenklinik Boon.
Klin Monbl Augenheilkd. 1989 Dec;195(6):368-70. doi: 10.1055/s-2008-1050057.
Semiquantitative fluorescein photometric studies were performed on the retinas of lyophilized rat eyes. Before injecting the fluorescein, the right, dyeless eyes were enucleated under anesthesia with ketamine hydrochloride. The left eyes were removed between 20 seconds and two hours after intravenous injection of fluorescein. Histologic sections of each of the posterior segments were measured fluorophotometrically. The photometric values of most of the fluorescein-dyed retinal sections were higher than those of the contralateral (dyeless) control eyes. With increasing duration of observation the differences between the sections which contained fluorescein and those which did not diminished, although some temporary and limited increases occurred. The higher extinction values of the specimens containing fluorescein are in agreement with the quantitative results of Grimes et al. (1982), who found fluorescein in all retinal layers. Anesthesia-induced acidoses cannot be ruled out may be considered minimal. The information value of quantitative and semiquantative results is certainly superior to qualitative results is certainly superior to qualitative findings.
对冻干大鼠眼球的视网膜进行了半定量荧光素光度研究。在注射荧光素之前，用盐酸氯胺酮麻醉后摘除右侧无染料的眼睛。在静脉注射荧光素后20秒至两小时之间摘除左侧眼睛。对每个后段进行组织学切片荧光光度测量。大多数荧光素染色的视网膜切片的光度值高于对侧（无染料）对照眼。随着观察时间的增加，含荧光素的切片与不含荧光素的切片之间的差异减小，尽管出现了一些暂时的、有限的增加。含荧光素标本的较高消光值与Grimes等人（1982年）的定量结果一致，他们在所有视网膜层中都发现了荧光素。不能排除麻醉引起的酸中毒，但其影响可能最小。定量和半定量结果的信息价值肯定优于定性结果。
