使用细胞指数富集的配体系统进化技术开发RNA适配体作为HER2(+)乳腺癌研究的分子探针。
Development of RNA aptamers as molecular probes for HER2(+) breast cancer study using cell-SELEX.
作者信息
Moosavian Seyedeh Alia, Jaafari Mahmoud Reza, Taghdisi Seyed Mohammad, Mosaffa Fatemeh, Badiee Ali, Abnous Khalil
机构信息
Biotechnology Research Center, Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Iran J Basic Med Sci. 2015 Jun;18(6):576-86.
OBJECTIVES
Development of molecules that specifically recognize cancer cells is one of the major areas in cancer research. Human epidermal growth factor receptor 2 (HER2) is specifically expressed on the surface of breast cancer cells. HER2 is associated with an aggressive phenotype and poor prognosis. In this study we aimed to isolate RNA aptamers that specifically bind to HER2 overexpressing TUBO cell line.
MATERIALS AND METHODS
Panel of aptamers was selected using cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX).
RESULTS
Binding studies showed that selected aptamers can identify TUBO cell line with high affinity and selectivity. Our preliminary investigation of the target of aptamers suggested that aptamers bind with HER2 proteins on the surface of TUBO cells.
CONCLUSION
We believe the selected aptamers could be useful ligands for targeted breast cancer therapy.
目的
开发能够特异性识别癌细胞的分子是癌症研究的主要领域之一。人表皮生长因子受体2（HER2）在乳腺癌细胞表面特异性表达。HER2与侵袭性表型和不良预后相关。在本研究中，我们旨在分离能特异性结合过表达HER2的TUBO细胞系的RNA适配体。
材料与方法
通过基于细胞的指数富集配体系统进化技术（细胞SELEX）筛选适配体文库。
结果
结合研究表明，筛选出的适配体能够以高亲和力和选择性识别TUBO细胞系。我们对适配体靶点的初步研究表明，适配体与TUBO细胞表面的HER2蛋白结合。
结论
我们认为筛选出的适配体可能是靶向乳腺癌治疗的有用配体。
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