Kucukdurmaz F, Kucukgergin C, Akman T, Salabas E, Armagan A, Seckin S, Kadıoglu A
Istanbul University, Istanbul Medical Faculty, Department of Urology, Istanbul, Turkey.
Istanbul University Istanbul Medical Faculty, Department of Biochemistry, Istanbul, Turkey.
Andrologia. 2016 May;48(4):374-9. doi: 10.1111/and.12455. Epub 2015 Jul 29.
Ischaemic priapism is characterised by hypoxia, hypercapnia and acidosis with resultant corporal fibrosis. Studies reported decreased erectile recovery after treatment of priapism longer than 36 h. However, a recent study revealed that half of patients with 3 days of priapism achieved recovery after T-shunt, although mechanism remains unclear. We aimed to investigate the effect of priapism duration on oxidative stress and antioxidant enzymes. Twenty-four male rats were divided into four groups. Group 1 served as control. Groups 2, 3 and 4 represented 1, 2 and 4 h, respectively, of priapism induced by vacuum device and rubber band placed at base of erect penis. After 30 min of reperfusion, penectomy and blood withdrawal were performed to investigate levels of malondialdehyde (MDA), protein carbonyl (PC), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx). Corporal MDA progressively increased with priapism duration (P = 0.01). Corporal SOD significantly differed between groups 1, 2 and 4. Also, there were significant differences in corporal GPx in groups 1 and 4 (P = 0.004) and groups 2 and 4 (P = 0.01). Corporal CAT was higher in group 4, but multivariable analysis revealed insignificant differences. Plasma MDA of the experimental groups was significantly higher than that of controls. There were no differences among groups in terms of other parameters. Increased antioxidant enzymes according to duration of priapism suggest that immediate treatment to relieve oxidative stress should be initiated in prolonged cases. However, further studies should be conducted to determine resistance mechanisms of the corpora to prolonged ischaemia.
缺血性阴茎异常勃起的特征是缺氧、高碳酸血症和酸中毒，并伴有海绵体纤维化。研究报告称，阴茎异常勃起持续超过36小时后，勃起功能恢复下降。然而，最近一项研究显示，阴茎异常勃起3天的患者中有一半在进行T型分流术后实现了恢复，尽管其机制尚不清楚。我们旨在研究阴茎异常勃起持续时间对氧化应激和抗氧化酶的影响。将24只雄性大鼠分为四组。第1组作为对照组。第2、3和4组分别代表通过真空装置和放置在勃起阴茎根部的橡皮筋诱导的阴茎异常勃起1小时、2小时和4小时。再灌注30分钟后，进行阴茎切除和采血，以研究丙二醛（MDA）、蛋白质羰基（PC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）的水平。海绵体MDA随阴茎异常勃起持续时间逐渐增加（P = 0.01）。第1、2和4组之间海绵体SOD有显著差异。此外，第1和4组（P = 0.004）以及第2和4组（P = 0.01）之间海绵体GPx也有显著差异。第4组海绵体CAT较高，但多变量分析显示差异不显著。实验组的血浆MDA显著高于对照组。在其他参数方面，各组之间没有差异。根据阴茎异常勃起持续时间增加抗氧化酶表明，对于病程较长的病例，应立即开始治疗以减轻氧化应激。然而，应进行进一步研究以确定海绵体对长时间缺血的抵抗机制。
