Munarriz Ricardo, Park Kwangsung, Huang Yue-Hua, Saenz de Tejada Iñigo, Moreland Robert B, Goldstein Irwin, Traish Abdulmaged M
Department of Urology, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
Urology. 2003 Oct;62(4):760-4. doi: 10.1016/s0090-4295(03)00484-9.
To assess the physiologic and biochemical changes resulting from ischemia and reperfusion. Effective therapy for ischemic priapism reestablishes corporal venous outflow and arterial inflow and results in increased corporal partial pressure of oxygen. Data are limited concerning reperfusion injury of ischemic erectile tissue associated with reactive oxygen species (ROS) and the potential role of ROS scavengers in the clinical therapy of ischemic priapism.
Anesthetized adult New Zealand white male rabbits (n = 7) were exposed to a low oxygen tension breathing gas to achieve hypoxia within the corpora cavernosa. This resulted in a mean systemic oxygen saturation of 60%. The pelvic nerve was electrically stimulated to induce penile erection, and the base of the erect penis was clamped. After varying durations of ischemia, the clamp was removed to allow reperfusion. We determined the intracavernosal oxygen tension, histologic changes, myeloperoxidase activity, and lipid peroxidation.
Corporal partial pressure of oxygen progressively decreased as the duration of priapism increased. A statistically significant increase was noted in myeloperoxidase activity and lipid peroxidation with corporal reperfusion. Polymorphonuclear leukocyte infiltration was documented in the ischemic reperfused tissue.
In the management of ischemic priapism, reperfusion causes erectile tissue injury owing to the presence of ROS. There is a need to investigate the utility of ROS scavengers and antioxidants in the management of ischemic priapism.
评估缺血和再灌注引起的生理和生化变化。缺血性阴茎异常勃起的有效治疗可重建海绵体静脉流出和动脉流入，并导致海绵体氧分压升高。关于与活性氧（ROS）相关的缺血性勃起组织的再灌注损伤以及ROS清除剂在缺血性阴茎异常勃起临床治疗中的潜在作用的数据有限。
对成年新西兰雄性白兔（n = 7）进行麻醉，使其吸入低氧分压的气体，以实现海绵体内缺氧。这导致全身平均氧饱和度为60%。电刺激盆腔神经以诱导阴茎勃起，然后夹住勃起阴茎的根部。在不同时长的缺血后，松开夹子以进行再灌注。我们测定了海绵体内氧分压、组织学变化、髓过氧化物酶活性和脂质过氧化。
随着阴茎异常勃起持续时间的增加，海绵体氧分压逐渐降低。再灌注时，髓过氧化物酶活性和脂质过氧化显著增加。在缺血再灌注组织中记录到多形核白细胞浸润。
在缺血性阴茎异常勃起的治疗中，再灌注由于ROS的存在而导致勃起组织损伤。有必要研究ROS清除剂和抗氧化剂在缺血性阴茎异常勃起治疗中的效用。
